2023-09-042023-09-042023-08-17FONTENELE, Thaís Moreira. Estudo do mecanismo de ação da atividade leishmanicida da miltefosina. 2023. 49 f. Trabalho de Conclusão de Curso (Bacharelado em Física Médica) - Instituto de Física, Universidade Federal de Goiás, Goiânia, 2023.http://repositorio.bc.ufg.br/handle/ri/23535Miltefosine (MT) is an internationally approved oral drug for the treatment of leishmaniasis, however, its mechanisms of action are not clear yet. In this study, we used electron paramagnetic resonance (EPR) spectroscopy associated with the spin label method to demonstrate that MT causes membrane rigidity in promastigotes of L. amazonensis after treatments of 24, 48 and 72 h. The EPR spectra were consistent with the occurrence of intense lipoperoxidation and oxidation of membrane proteins. These results agree with reported works showing that MT also causes changes in the mitochondrial membrane potential, as well as several compounds that also cause membrane rigidity in the Leishmania parasite. As previous studies have shown that MT causes great fluidity in the parasite's membrane after two hours of treatment, the results of this work suggest that the primary action of MT is on the plasmatic membrane, with probable alteration in the ionic balance, like ivermectin that alters the electrical potential on the parasite membrane and causes membrane rigidity as has been reported.porAcesso AbertoLeishmaniaMiltefosinaEspectroscopia de ressonância paramagnéticaMarcador de spinFluidez de membranaMiltefosineParamagnetic resonance spectroscopySpin markerMembrane fluidityTCC