2019-06-032019-06-032012TOSCANO, Daniela Araújo; ÁVILA, Marcos Pereira de; CHALITA, Maria Regina Catai. Reproducibility of peripapillary retinal nerve fiber layer thickness measurements using Spectral Domain OCT in Brazilian patients. Arquivos Brasileiros de Oftalmologia, Rio de Janeiro, v. 75, n. 5, p. 320-323, 2012.0004-2749http://repositorio.bc.ufg.br/handle/ri/17599Purpose: To evaluate the reproducibility of peripapillary retinal nerve fiber layer (RNFL) thickness measurements in normal eyes and eyes with glaucoma using spectral domain optical coherence tomography (SDOCT). Methods: One eye of 79 normal and 72 glaucoma patients was analyzed. All patients underwent a complete ophthalmological examination, including visual acuity testing; intraocular pressure, slit-lamp examination, indirect ophthalmos- copy; and the glaucoma group underwent achromatic perimetry with the 24-2 SITA Fast Humphrey Field Analyzer. All patients’ eyes were scanned using the spectral domain optical coherence tomography - Spectralis ® and one of them was cho­­­sen randomly. Three con­­­­­secutive circular B-scan centered at the optic disc were performed in one visit. Results: The intraclass correlation coefficient (ICC), coefficient of variation and test-retest variability for the mean retinal nerve fiber layer thickness were respec- tively: 0.94, 2.56% and 4.85 μm for the normal group and 0.93, 4.65% and 6.61 μm for the glaucomatous group. The intraclass correlation coefficient for retinal nerve fiber layer thickness in all quadrants were all excellent in both groups, with the superior quadrant having the highest ICCs (0.964) in glaucomatous eyes and nasal quadrant measurements having the lowest (0.800), but still excellent in eyes without glaucoma. The coefficient of variation was between 2.56% - 8.74% and between 4.65% - 11.44% in normal and glaucomatous group respectively. The test-retest variability was between 4.85 μm and 11.51 μm in the normal group and between 6.61 μm and 14.24 μm in the glaucomatous group. The measurements in glauco- matous eyes were more variable than normal eyes. Conclusions: Spectral domain optical coherence tomography showed excellent reproducibility with regard to retinal nerve fiber layer thickness measurements in normal and glaucomatous eyes.engAcesso AbertoDiagnostic techniquesReproducibility of resultsOphthalmologicalNerve fibersGlaucoma/diagnosisOptic nerve/pathologyTomographyRetinal ganglion cellsOptical coherence/methodsTécnicas de diagnóstico oftalmológicoReprodutibilidade dos testesGlaucoma/diagnosticoTomografia de coerência óptica/métodosCélulas ganglionares da retinaFibras nervosasNervo óptico/patologiaArtigo10.1590/S0004-27492012000500005