2018-01-232018-01-232011OLIVEIRA, Benito Juarez Nunes Alves de; SOUZA, Luiz Augusto de; EURIDES, Duvaldo; SILVA, Luiz Antônio Franco da; BAUNGARTEN, Letícia Binda; BUSNARDO, Camila Araújo. Aislamiento, viabilidad y rendimiento de células mononucleares de médula ósea de conejos. Acta Bioquímica Clínica Latinoamericana, La Plata, v. 45, n. 1, p. 113-118, 2011.e- 1851-61140325-2957http://repositorio.bc.ufg.br/handle/ri/13481The aim of this study was to establish a cellular isolation protocol and evaluate the yield and viability of bone marrow mononuclear cells in rabbits. Thirty-two male adult New Zealand rabbits were used with 3.06 ± 0.24 Kg mean weight. A volume of 2.0 mL bone marrow was aspirated from the humerus of each animal. Samples were diluted in Dulbecco phosphate buffered saline (DPBS) and submitted to density gradient using Ficoll-Ipaque (1.077 g/mL) at a 2:1 rate. Subsequently, the material was centrifuged at 495g during 30 minutes, at 15 oC. The yield and cell viability were determined in Neubauer haemocytometer chamber, using trypan blue vital staining. A mean yield [±SD] of 6.65 [±4.18] x106 cells/mL was obtained and the mean viability [±SD] was 92.66 [±4.35] %. The protocol using density gradient was effective for the isolation, yield and viability of bone marrow mononuclear cells in rabbits in order to use them in cell therapy.spaAcesso AbertoCélulas madre mesenquimalesMédula óseaAislamientoConejosMesenchymal stem cellsBone marrowIsolationRabbitsArtigo