2020-09-302020-09-302020-07-07OLIVEIRA, Fernanda Miguel de. Avaliação do teor de fenóis totais, flavonoides totais e hibalactona nas partes aéreas de Hydrocotyle umbellata L. (Araliaceae). 2020. 34 f. Trabalho de Conclusão de Curso (Graduação) – Faculdade de Farmácia, Universidade Federal de Goiás, Goiânia, 2020.http://repositorio.bc.ufg.br/handle/ri/19119Hydrocotyle umbellata L., popularly known as "acariçoba", is a species belonging to the family Araliaceae, native to the American continent. The anxiolytic, antinociceptive and antiinflammatory activities were demonstrated for the subterraneous parts of H. umbellata and these activities were in part attributed to the presence of the lignan hibalactone. The objective of this work was to carry out quality control studies and determine the content of total phenols, total flavonoids and hibalactone in the aerial parts of H. umbellata. An appreciable amount of phenolic compounds and flavonoids was observed (3,54% and 3,04%). The co-validation of the HPLC method for quantification of hibalactone proved to be selective, linear and accurate, being useful for its quantification in the plant drug of aerial parts of H. umbellata. The best conditions for the ultrasound extraction of hibalactone, obtained from the Box-Benhken design associated with the response surface methodology were: solid-liquid ratio of 0.9 g/10 mL, 67% ethanol content (v/v) and extraction temperature of 64 °C. The content of hibalactone found in the aerial parts was lower than that reported for the subterraneous parts, however more studies must be carried out to investigate the possibility of using therapeutically the subterraneous and aerial parts of the plant.porAcesso AbertoAcariçobaControle de qualidadeMetabólitos secundáriosCLAEMetodologia de superfície de respostaAcariçobaQuality controlSecondary metabolitesHPLCResponse surface methodologyTCC