2022-12-152022-12-152022-08-09SILVA, Thayanara Lorrane Alves da. Desenvolvimento e validação de método eco-friendly por UV para avaliação de marbofloxacino em comprimidos. 2022. 87 f. Trabalho de Conclusão de Curso (Bacharelado em Farmácia) - Faculdade de Farmácia, Universidade Federal de Goiás, Goiânia, 2022.http://repositorio.bc.ufg.br/handle/ri/21665Fluoroquinolones are a class of antimicrobials, which stand out as an important alternative for the treatment of infections caused by bacteria with a broad spectrum of action. Analytical methods found in the literature related to marbofloxacin (MAR, second-generation fluoroquinolone) show a predominance in the use of toxic organic solvents, which promote the generation of waste, potentially harmful to the environment and the operator. Therefore, na analytical method was proposed for the quantitative analysis of MAR in tablets, by means of spectrophotometry in the ultraviolet (UV) region. Thus, an eco-friendly method was developed and validated aiming speed, low cost, use of less toxic solvents, reducing harmful actions to operators, as well as waste to the environment, in accordance with the principles of Green Analytical Chemistry. The method was developed and validated for the quantification of MAR in tablets by spectrophotometry in the UV region at 296 nm, using quartz cuvettes and purified water: ethanol (80:20, v/v) as a diluent. The method was linear at the concentration of 3-9 μg/mL, with a correlation coefficient of 0.9994 (standard), accurate with a relative standard deviation (RSD) of 1.20 % for the intraday level, 1.68 % for the interday level is 3.26 % for the interanalyst level, exact with an average recovery of 100.39 %, selective in the comparison of standard and sample MAR and robust against changes in wavelength and in the proportion of ethanol used in the preparation of stock solutions. The average content of MAR in tablets, using the validated method, was 103.67 %. Thus, the method can be applied for quantitative assessment of MAR in tablets in an ecologically sustainable, reliable and effective way, being a green analytical option for chemical-pharmaceutical laboratories.porAcesso AbertoControle de qualidadeValidação de método analíticoQuímica analítica verdeMarbofloxacinoEspectrofotometria no UVQuality controlAnalytical method validationGreen analytical chemistryMarbofloxacinUV spectrophotometryTCC