Tentativa de isolamento primário de t. cruzi de pacientes crônicos de doença de chagas por hemocultura — agentes bloqueadores
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1983-08
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Ruy de Souza Lino Junior
Resumo
ot ('hagas' disease were earried out in
four separate trials. The 250 cases were
divided into four groups consisting respectivelly
of 100, 100. ;i() and 20 patients.
In the first three experiments the
blood for hemoculture was taken with
heparin. One drop of the anticoagulant
for 5ml of blood \vas used. Ml blood sarnples
were inoculated on LIT culture
médium, l.'!0 samples w pré additionally
inoeulated on médium 19() plus 30%
fetal bovine serum (FHS 80%) and on
the former culture médium to which
total extract of the bug (Triatoma infeslans)
vvas added. Ali these 230 samples
were ineubated for 60 days. at 26<X; and
were examined evcry 15 days. In the last
experirneiit. lhe hemoculture was also
earried out on 1,1'L médium but in these
20 cases ali blood samples were taken
with COTA: 10 of these samples were
washed hefore inoculalion. the other 10
samples were not washed, Incubation was
earried out at 26°C was maintained for
at least 90 days. The examinations
showed negative results in the first 230
cases of the first three experiments. In
the last experiment where blood samples
had l te e n colleeted with FJ)TA. we observed
positive results in two out of the teu
samples in which blood was washed and
in si x out of the ten samples in which
blood was not washed. Tinia heparin
showed to be an important blocking
age ti t in the isolation of T. cruzi. The
type of anticoagulant used and the time
of observatíon of cnltures constitute
very important factors for the sueeess or
failure in isolation of T. cruzi.
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BARBOSA, William; CZEREWUTA, Ana Cândido; OLIVEIRA, Raquel Lopes de. Tentativa de isolamento primário de t. cruzi de pacientes crônicos de doença de chagas por hemocultura — agentes bloqueadores. Revista de Patologia Tropical, Goiânia, v. 12, n.2, p. 155-163, mai./ago. 1983. Disponível em: < https://www.revistas.ufg.br/iptsp/article/view/21293/12502>.