Marker-assisted selection for the rice blast resistance gene Pi-ar in a backcross population
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Data
2010
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Resumo
A doubled-haploid (DH) population, obtained by anther culture of F 1 plants from a cross between a highly
susceptible rice cultivar Lijiangxintuanheigu and the resistant somaclone (SC09), of the cultivar Araguaia, was used to identify
RAPD markers linked to the blast resistance gene Pi-ar. The 86 DH plants, inoculated with the race IB-9 of Magnaporthe
oryzae, segregated in 1:1 ratio of resistant and susceptible plants. Of the 67 primers used 31 produced DNA profiles that
differentiated resistant and susceptible bulks as well as the parental cultivars. The resistance gene was found linked to the
primer OPS16 2072 (‘AGGGGGTTCC’) at a distance of 3.6 cM. The selection efficiency of this primer was assessed in a BC 3 F 1
population derived from another cross between a susceptible cultivar IAC 201 and SC09. The marker OPS16 showed
efficiency of 86.9%, when six resistant and two susceptible plants were considered as negatives in RAPD analysis.
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Anther culture, Oryza sativa, Double haploid, Magnaporthe oryzae, Cultura de antera, Molecular markers, Duplo haplóide, Marcadores moleculares, Magnaporthe oryzae, Oryza sativa
Citação
ARAÚJO, Leila Garcês de; PRABHU, Anne Sitarama; PEREIRA, Pedro Antonio Arraes; SILVA, Gisele Barata da. Marker-assisted selection for the rice blast resistance gene Pi-ar in a backcross population. Crop Breeding and Applied Biotechnology, Viçosa, v. 10, p. 23-31, 2010.