Comparação entre técnicas de coloração de raízes de soja infectadas por Heterodera glycines, Meloidogyne incognita e Pratylenchus brachyurus
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Data
2015
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Resumo
Experiment was conducted in a greenhouse with the purpose to evaluate the
effectiveness of two root staining techniques on soybean cultivar Emgopa 313, infected
by the three main nematodes of the crop: Heterodera glycines, Meloidogyne incognita
and Pratylenchus brachyurus. Three evaluations were performed at 10, 17 and 25 days
after planting in naturally infested soil. The technique using bleaching with NaOCl and
acid fuccin and the technique that employs lacto-glycerol and acid fuccin were both
effective in staining nematodes, allowing its observation in the root tissue. Because of
longer duration of the life cycle of P. brachyurus it was not possible to view these
nematodes in root the tissue at the first evaluation using the technique of bleaching and
staining with acid fuccin, and at the second evaluation using both techniques. For young
tissues the technique that employs bleaching is more indicated to facilitate the
nematode observation, since it uses less stain which helps the process of discoloration.
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Glicine max, nematóide de cisto da soja, nematóide de galhas, nematóide das lesões radiculares, Glicine max, root-knot nematode, soybean cyst nematode, lesion nematode
Citação
ARAÚJO, Fernando Godinho de; BARBOSA, Kássia Aparecida Garcia; SANTOS, Leonardo de Castro; TEIXEIRA, Renato Andrade; ROCHA, Mara Rubia da. Comparação entre técnicas de coloração de raízes de soja infectadas por Heterodera glycines, Meloidogyne incognita e Pratylenchus brachyurus. Multi-Science Journal, Urutaí, v. 1, n. 1, p. 100-105, 2015.