Macrophage interaction with Paracoccidioides Brasiliensis yeast cells modulates fungal metabolism and generates a response to xidative stress
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2015
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Macrophages are key players during Paracoccidioides brasiliensis infection. However, the
relative contribution of the fungal response to counteracting macrophage activity remains
poorly understood. In this work, we evaluated the P. brasiliensis proteomic response to macrophage
internalization. A total of 308 differentially expressed proteins were detected in P.
brasiliensis during infection. The positively regulated proteins included those involved in
alternative carbon metabolism, such as enzymes involved in gluconeogenesis, beta-oxidation
of fatty acids and amino acids catabolism. The down-regulated proteins during P. brasiliensis
internalization in macrophages included those related to glycolysis and protein
synthesis. Proteins involved in the oxidative stress response in P. brasiliensis yeast cells
were also up-regulated during macrophage infection, including superoxide dismutases
(SOD), thioredoxins (THX) and cytochrome c peroxidase (CCP). Antisense knockdown
mutants evaluated the importance of CCP during macrophage infection. The results suggested
that CCP is involved in a complex system of protection against oxidative stress and
that gene silencing of this component of the antioxidant system diminished the survival of P.
brasiliensis in macrophages and in a murine model of infection.
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PARENTE-ROCHA, Juliana Alves et al. Macrophage interaction with Paracoccidioides Brasiliensis yeast cells modulates fungal metabolism and generates a response to xidative stress. Plos One, San Francisco, v. 10, n. 9, e0137619, 2015.