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Tipo do documento: Artigo
Título: Antioxidant effect of 4-nerolidylcatechol and α-tocopherol in erythrocyte ghost membranes and phospholipid bilayers
Autor: Fernandes, Kelly de Souza
Silva, Adolfo Henrique de Moraes
Mendanha Neto, Sebastião Antônio
Rezende, Kennia Rocha
Alonso, Antonio
Abstract: 4-Nerolidylcatechol (4-NC) is found in Pothomorphe umbellata root extracts and is reported to have a topical protective effect against UVB radiation-induced skin damage, toxicity in melanoma cell lines, and antimalarial activity. We report a comparative study of the antioxidant activity of 4-NC and a-tocopherol against lipid peroxidation initiated by two free radical-generating systems: 2,29-azobis(2-aminopropane) hydrochloride (AAPH) and FeSO4/H2O2, in red blood cell ghost membranes and in egg phosphatidylcholine (PC) vesicles. Lipid peroxidation was monitored by membrane fluidity changes assessed by electron paramagnetic resonance spectroscopy of a spin-labeled lipid and by the formation of thiobarbituric acid-reactive substances. When lipoperoxidation was initiated by the hydroxyl radical in erythrocyte ghost membranes, both 4-NC and a-tocopherol acted in a very efficient manner. However, lower activities were observed when lipoperoxidation was initiated by the peroxyl radical; and, in this case, the protective effect of a-tocopherol was lower than that of 4-NC. In egg PC vesicles, malondialdehyde formation indicated that 4-NC was effective against lipoperoxidation initiated by both AAPH and FeSO4/H2O2, whereas atocopherol was less efficient in protecting against lipoperoxidation by AAPH, and behaved as a pro-oxidant for FeSO4/H2O2. The DPPH (2,2-diphenyl-1-picrylhydrazyl) free-radical assay indicated that two free radicals were scavenged per 4-NC molecule, and one free radical was scavenged per a-tocopherol molecule. These data provide new insights into the antioxidant capacity of 4-NC, which may have therapeutic applications for formulations designed to protect the skin from sunlight irradiation.
Palavras-chave: Electron paramagnetic resonance
Spin label
Lipid peroxidation
País: Brasil
Unidade acadêmica: Instituto de Física - IF (RG)
Citação: FERNANDES, K. S.; SILVA, A. H. M.; MENDANHA, S. A.; REZENDE, K. R.; ALONSO, A. Antioxidant effect of 4-nerolidylcatechol and α-tocopherol in erythrocyte ghost membranes and phospholipid bilayers. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 46, n. 9, p. 780-788, Sept. 2013.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1590/1414-431X20132940
Identificador do documento: 10.1590/1414-431X20132940
Data de publicação: 2013
Aparece nas coleções:IF - Artigos publicados em periódicos

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