Optimizing the parasitological diagnosis of congenital toxoplasmosis
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Data
2013-12
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Resumo
Early diagnosis of Congenital Toxoplasmosis is highly important,
since it can make the treatment possible and reduces sequela for the infant.
Serological diagnosis alone cannot be accurate when it does not identify the
IgA, IgM or IgG antibodies of low avidity, which do not cross the placent
barrier. Therefore, parasitemic identification is important to be carried out by
demonstrating the parasite in the peritoneal exsudatum of mice inoculated
with suspected biological material, however such method is little sensitive
and too much time-consuming. This research study aims at optimizing mice
inoculation through serological screening, and encephalic histopathology, in
order to identify whether there was contamination. Out of 138 fetuses and/or
newborn samples taken from pregnant women positive for active toxoplasmosis inoculated intraperitoneally in mice, only 5 showed positive through
parasite demonstration in the peritoneal exsudatum. Histopathology showed
the agent in 45 cases and in 67 the presence of anti-toxoplasmas antibodies
in the mice bloodstream by using indirect immunofluorescence technique.
Mice serology and encephalic histopathology in addition to reducing the
amount of time necessary for the outcomes from 120 to 60 days increased the
positivity of 3.6% to 50.4% and 33.8%, respectively.
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Palavras-chave
Congenital toxoplasmosis, Experimental serology, Parasitological diagnosis, Mice inoculation, Toxoplasmose congênita, Sorologia experimental, Diagnóstico parasitológico, Inoculação em camundongos
Citação
SILVA, Marcos Gontijo da; Avelino, Mariza Martins; AMARAL, Waldemar Naves do; CASTRO, Ana Maria de. Optimizing the parasitological diagnosis of congenital toxoplasmosis. Universitas: ciências da saúde, Brasília, v. 11, n. 2, p. 75-81, July/Dec. 2013.