Characterization of Paracoccidioides brasiliensis isolates by random ampified polymorphic DNA analysis
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1995-02
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We initially used 25 different random primers in order to test their ability to generate random amplified
polymorphic DNA fragments from the dimorphic human pathogenic fungus Paracoccidioides brasiliensis. From
the tested primers we chose five to distinguish between seven isolates of this microorganism. The DNA
amplification patterns allowed clear differentiation of the seven isolates into two distinct groups with only 35%
genomic identity. One of these groups contained two subgroups with 81% genetic similarity. The random
amplified polymorphic DNA analysis method proved to be a good tool for analyzing and comparing different
genomes of P. brasiliensis isolates.
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SOARES, C. M. A.; MADLUN, E. E. W. I. Mollinari; SILVA, S. P. da; PEREIRA, M.; FELIPE, M. S. S. Characterization of Paracoccidioides brasiliensis isolates by random ampified polymorphic DNA analysis. Journal of Clinical Microbiology, Washington, v. 33, n. 2, p. 505-507, Feb. 1995.