Susceptibility testing of Candida albicnas isolated from oropharyngeal mucosa of HIV+ patients to fluconazole, amphotericin B and caspofungin. Killing kinetics of caspofungin and amphotericin B against fluconazole resistant and susceptible isolates
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Data
2009
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Resumo
A clear understanding of the pharmacodynamic properties of antifungal agents is important for the adequate
treatment of fungal infections like candidiasis. For certain antifungal agents, the determination of Minimal
Fungicidal Concentration (MFC) and time kill curve could be clinically more relevant than the determination
of the Minimal Inhibitory Concentration (MIC). In this study, MIC and MFC to fluconazole, amphotericin B
and caspofungin against C. albicans isolates and the killing patterns obtained with caspofungin and
amphotericin B against susceptible and resistant strains to fluconazole were determined. The results of MICs
showed that all C. albicans isolates were highly susceptible to amphotericin B, but two isolates were
fluconazole resistant. The comparative analysis between MIC and MFC showed that MFC of fluconazole was
fourfold higher than MIC in 41.9% of the C. albicans isolates. Same values of MFC and MIC of amphotericin
B and caspofungin were found for 71% of the isolates. Correlation between time kill curves and MFC of
amphotericin B and caspofungin against all 4 isolates tested was observed. The caspofungin killing effect
was more evident at MFC in 6 hours of incubation than at MIC in this time, suggesting dependence of
concentration. The similarity of results of time-kill curve and MFC values indicate that determination of MFC
is an alternative for the detection of the fungicidal activity of these drugs.
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Antifungal susceptibility, Candida albicans, Time kill curves, Suscetibilidade antifúngica, Candida albicans, Curva do tempo de morte
Citação
LEMOS, Janine de Aquino; COSTA, Carolina Rodrigues; ARAÚJO, Crystiane Rodrigues de; SOUZA, Lúcia Kioko Hasimoto e; SILVA, Maria do Rosário Rodrigues. Susceptibility testing of Candida albicnas isolated from oropharyngeal mucosa of HIV+ patients to fluconazole, amphotericin B and caspofungin. Killing kinetics of caspofungin and amphotericin B against fluconazole resistant and susceptible isolates. Brazilian Journal of Microbiology, São Paulo, v. 40, n. 1, p. 163-169, Jan./Mar. 2009.