1H HR-MAS NMR and S180 cells: metabolite assignment and evaluation of pulse sequence

Resumo

High resolution magic angle spinning 1H nuclear magnetic resonance spectroscopy (HR-MAS NMR) is a useful technique for evaluation of intact cells and tissues. However, optimal NMR parameters are crucial in obtaining reliable results. To identify the key steps for the optimization of HR-MAS NMR parameters, we assessed different pulse sequences and NMR parameters using sarcoma 180 (S180) cells. A complete assignment of the metabolites of S180 is given to assist future studies.

Descrição

Palavras-chave

HR-MAS NMR, Sarcoma 180, S180, CPMG

Citação

OLIVEIRA, Aline L. de et al. 1H HR-MAS NMR and S180 cells: metabolite assignment and evaluation of pulse sequence. Journal of the Brazilian Chemical Society, Campinas, v. 25, n. 6, p. 1135-1142, Apr. 2014