Isolation and quantitative HPLC-PDA analysis of lupeol in phytopharmaceutical intermediate products from Vernonanthura ferruginea (Less.) H. Rob.
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Data
2012
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Descrição
Prior to obtain a standardized dried extract from V. ferruginea, lupeol was first time isolated from leaves and used as chemical maker.
An analytical method using HPLC-PDA for lupeol determination in V. ferruginea intermediate products was developed using a C8
reverse-phase column, acetonitrile-acetic acid (99.99:0.01, v/v) as mobile phase at 0.8 mL min -1 , oven temperature at 23‐25 °C, sample
injection volume at 30 μL and detection at 210 nm. The method presented linearity from 10 to 160 μg mL -1 , accuracy, precision,
robustness and suitable sensitivity proving to be a useful tool to the obtainment process of lupeol standardized dried extracts of
V. ferruginea.
Palavras-chave
Quality control, Standardized dried extract, Triterpene
Citação
OLIVEIRA, Ezequiane Machado Silva et al. Isolation and quantitative HPLC-PDA analysis of lupeol in phytopharmaceutical intermediate products from Vernonanthura ferruginea (Less.) H. Rob. Química Nova, São Paulo, v. 35, n. 5, p. 1041-1045, 2012.