Determinação de taninos e metilxantinas no guaraná em pó (Paullinia cupana Kunth, Sapindaceae) por cromatografia líquida de alta eficiência
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Data
2010-12
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Resumo
The seeds of Paullinia cupana Kunth, Sapindaceae, are used as a
stimulant and the main chemical constituents are the methylxanthines and tannins, as
catechin and epicatechin. This study aimed to investigate a method by high performance
liquid chromatography (HPLC) to quantify both caffeine, theophylline, theobromine,
catechin and epicatechin in powdered of P. cupana. The values obtained were compared
with the results of the spectrophotometric method of the Brazilian Pharmacopoeia IV ed.
For the chromatographic method, the levels of methylxanthines and total tannins were
4.04% and 1.48%, respectively. The levels of methylxanthines and total tannins obtained
by the spectrophotometric method, were 4.88% and 4.05%, respectively. It is believed
that, under our experimental conditions, the spectrophotometric method was not specific
and quantified others phenolic substances present in the P. cupana. Therefore we suggest
the use of this chromatographic method in quality control of P. cupana powder, to be
accurate, sensitive, specific and fast.
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Controle de qualidade, CLAE, Planta medicinal, Guaraná, Cafeína, Catequina, Quality control, Catechin, Medicinal plant, Caffeine, HPLC
Citação
SOUSA, Sandra A. et al. Determinação de taninos e metilxantinas no guaraná em pó (Paullinia cupana Kunth, Sapindaceae) por cromatografia líquida de alta eficiência. Revista Brasileira de Farmacognosia, Curitiba, v. 20, n. 6, p. 866-870, dez. 2010.