Assessment of the mutagenic, antimutagenic and cytotoxic activities of ethanolic extract of araticum (Annona crassiflora Mart. 1841) by micronucleus test in mice
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2008-02
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Resumo
A typical Brazilian plant, araticum (Annona crassiflora Mart.), is widely used in humans as therapeutic medicine to
treat several diseases such as diarrhea, rheumatism and syphilis. It contains acetogenins which present cytotoxic, anti-
tumogenic, and antiparasitic properties. In this study, mutagenic, antimutagenic and cytotoxic effects of araticum leaves
ethanolic extract were evaluated by micronucleus test in mice. To evaluate the mutagenic activity, animals were treated
with ethanolic extract of araticum (EEA) using 10, 20, 50, 100 and 160 mg.kg –1 . For all doses, micronucleated polychro-
matic erythrocytes (MNPCE) frequency was evaluated at 24, 48 and 72 hours after treatment. To evaluate the antimuta-
genic activity, animals were treated with 10, 20, 50 and 100 mg.kg –1 of EEA and 4 mg.kg –1 of MMC simultaneously. The
frequency of MNPCE was evaluated 36 hours after exposure. Cytotoxicity was evaluated by the polychromatic and nor-
mochromatic erythrocytes ratio (PCE/NCE). In the mutagenicity assessment, all doses of EEA resulted in no significant
increase of MNPCE (P > 0.05), compared to solvent- control group. Regarding administration time, no significant dif-
ference among three evaluation periods was observed (P > 0.05). Such results indicate that EEA did not exert mutagenic
activity. Cytotoxicity was evident in doses of 50, 100 and 160 mg.kg –1 at 24 and 48 hours after exposure. Concerning
antimutagenicity, except the 10 mg.kg –1 co-administered with 4 mg/kg of MMC, all doses reduced significantly the fre-
quency of MNPCE compared to the positive control group (P < 0.05). These results, therefore, indicate an antimutagenic
activity of the EEA. Cytotoxicity was significantly increased (P < 0.01) at 100 mg.kg –1 EEA doses co-administered with
4 mg.kg –1 of MMC.
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Annona crassiflora, Cytotoxicity, Araticum, Mutagenicity, Antimutagenicity, Annona crassiflora, Araticum, Antimutagenicidade, Citotoxicidade, Mutagenicidade
Citação
VILAR, J. B. et al. Assesment of the mutagenic, antimutagenic and cytotoxic activities of ethanolic extract of araticum ( Annona crassiflora mart. 1841) by micronucleus test in mice. Brazilian Journal of Biology, São Carlos, v. 68, n. 1, p. 149-155, Feb. 2008.