Characterization of the dry bean polygalacturonase-inhibiting protein (PGIP) gene family during Sclerotinia sclerotiorum (Sclerotiniaceae) infection
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Data
2010
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Resumo
Polygalacturonase-inhibiting proteins are leucine-rich
repeat proteins that inhibit fungal endopolygalacturonases. The inter-
action of polygalacturonase-inhibiting protein with endopolygalac-
turonases limits the destructive potential of endopolygalacturonases
and may trigger plant defense responses induced by oligogalacturo-
nides. We examined the expression of fungal pg and plant Pvpgip
genes in bean (Phaseolus vulgaris) stems infected with Sclerotinia
sclerotiorum to determine whether any of them are associated with the
infection process. Transcriptional analysis was carried out by means
of semi-quantitative reverse transcription PCR or real-time PCR. The
sspg1 gene was highly expressed during infection; sspg3 was regulat-
ed during the later phases of infection; sspg5 was more uniformly ex-
pressed during infection, whereas sspg6 was only weakly expressed.
During the course of infection, Pvpgip1 transcripts were not detected
at early stages, but they appeared 72 h post-inoculation. High levels
of Pvpgip2 expression were observed during the initial phase of infection; the transcript peaked by 48 h post-inoculation and declined by
72 h post-inoculation. Pvpgip3 expression increased strongly at 96
h post-inoculation. Pvpgip4 was constantly present from 24 h post-
inoculation until the end of the experiment. However, we detected
higher levels of the Pvpgip4 transcript in the necrotic lesion area
than in plants that had been mechanically wounded. Remarkably,
only Pvpgip4 appeared to be moderately induced by mechanical
wounding. These results provide evidence that endopolygalacturo-
nases contribute to the infection process during host colonization by
promoting the release of plant cell oligogalacturonides, which are
powerful signaling molecules and may also activate plant defenses,
such as polygalacturonase-inhibiting proteins.
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Palavras-chave
Endopolygalacturonase, Sclerotinia sclerotiorum, Phaseolus vulgaris, Polygalacturonase-inhibiting protein, White mold
Citação
OLIVEIRA, M. B. et al. Characterization of the dry bean polygalacturonase-inhibiting protein (PGIP) gene family during Sclerotinia sclerotiorum (Sclerotiniaceae) infection. Genetics and Molecular Research, Ribeirão Preto, v. 9, n. 2, p. 994-1004, 2010.