Molecular identification of Paracoccidioides brasiliensis by PCR amplification of ribossomal DNA
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2000-08
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We have amplified and sequenced the 5.8S and 28S ribosomal DNA genes and intergenic regions of
Paracoccidioides brasiliensis, strain Pb01. Using primers specifically designed for both ribosomal DNA regions,
we were able to discriminate between P. brasiliensis and other human pathogenic fungi by PCR. The use of this
molecular marker could be important for paracoccidiodomycosis diagnosis and ecological and molecular
epidemiological studies of P. brasiliensis in Latin America.
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MOTOYAMA, Andrea B. et al. Molecular identification of Paracoccidioides brasiliensis by PCR amplification of ribossomal DNA. Journal Of Clinical Microbiology, Washington, v. 38, n. 8, p. 3106-3109, Aug. 2000.