Determination of fluconazole in serum and amniotic fluid of rats by gaschromatography/ mass spectometry (GC/MS)
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Data
2005-06
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Resumo
Rats treated with oral dose of 100 mg/kg of fluconazole during
pregnancy had their serum and amniotic fluid quantified for this
drug using a GC/MS method. Fluconazole was extracted with ethyl
acetate from samples and analysed by a GC-MS Shimadzu QP5050A
system using a CBP-5 fused silica capillary column. Tioconazole
was used as internal standard. Calibration curve was linear within
the range 10.0 – 300.0 μg/mL. The limit of quantification was
0.1 μg/mL and no interference was observed in the blank serum
and amniotic liquid. The mean concentrations of the drug in the
serum and amniotic fluid were 206.01 ± 105.25 μg/mL and 125.34
± 65.24 μg/mL, respectively. This procedure showed to be sensitive
and efficient enough for the use in teratogenic studies of fluconazole
and other azole drugs.
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Palavras-chave
Fluconazole, GC/MS, Serum, Amniotic fluid, Fluconazol, CG/EM, Soro, Líquido amniótico
Citação
LIMA, Dione Marçal et al. Determination of fluconazole in serum and amniotic fluid of rats by gas-chomatography-mass spectrometry (GC/MS). Revista Brasileira de Ciências Farmacêuticas, São Paulo, v. 41, n. 2, p. 223-227, Apr./June 2005.