Detecção do gene mecA em estafilococos coagulase negativa resistentes à oxacilina isolados da saliva de profissionais da enfermagem

Resumo

Coagulase-negative staphylococci are frequently associated with nosocomial infections, and healthcare professionals can be reservoirs and spread them in hospitals and in the community. The aim of this study was to identify species of coagulase-negative staphylococci isolated from the saliva of nursing professionals, determine the resistance profile and detect the mecA gene. One hundred coagulase-negative staphylococci were selected: 41 were identified as Staphylococcus epidermidis, 25 as Staphylococcus saprophyticus, 18 as Staphylococcus haemolyticus, eight as Staphylococcus cohnii, four as Staphylococcus lugdunenses, three as Staphylococcus capitis and one as Staphylococcus simulans. Of these, 32% presented oxacillin resistance, 84.4% mupirocin resistance and 32% cefoxitin resistance, and all were vancomycin sensitive. Among the oxacillin-resistant coagulase-negative staphylococci, 93.7% developed in oxacillin agar (6µg/ml) and the mecA gene was detected in 75%. The results indicate that higher investments should be directed towards identifying coagulase-negative staphylococcus species in healthcare institutions and in the community.

Descrição

Palavras-chave

Gene mecA, Estafilococos coagulase negativa, Equipe de Enfermagem, Saliva, Resistência, mecA gene, Coagulase-negative staphylococci, Nursing team, Saliva, Resistance

Citação

ROSA, Juliana de Oliveira; MOURA, Josely Pinto de; PALOS, Marinésia Aparecida Prado; GIR, Elucir; REIS, Cleômenes; KIPNIS, André; CANINI, Sílvia Rita Marin da Silva; BELISSIMO-RODRIGUES, Fernando; PIMENTA, Fabiana Cristina. Detecção do gene mecA em estafilococos coagulase negativa resistentes à oxacilina isolados da saliva de profissionais da enfermagem. Revista da Sociedade Brasileira de Medicina Tropical, Uberaba, v. 42, n. 4, p. 398-403, 2009.