In vitro skin penetration of clobetasol from lipid nanoparticles: drug extraction and quantitation in different skin layers
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Data
2012-12
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Resumo
Clobetasol propionate (CP) is a potent topical corticosteroid that causes several cutaneous and systemic
side effects. In the present work, CP was encapsulated in nanostructured lipid carriers (NLCs) to increase
drug retention in the outer skin layers and improve the safety of topical therapy. NLCs were prepared
using a microemulsion technique with a mixture of lecithin, taurodeoxycholate, stearic acid, and oleic
acid. In vitro penetration studies were performed in a modified Franz-type diffusion cell, and porcine
ears were used as a model of human skin. A simple and sensitive liquid chromatographic method was
developed and validated for clobetasol determination in different skin layers. NLCs presented uniform
size distribution, high zeta potentialand entrapment efficiency values (> 98%). The analytical procedure
was validated according to FDA guidelines. Clobetasol recoveries from skin samples were higher than
85%, with no interference of skin components and NLC ingredients. In experiments, after 6 h, a higher
drug accumulation in the stratum corneum arising from NLCs compared to aqueous CP solution was
observed. Thus, the NLCs demonstrated high potential for targeting CP to the skin and ensuring drug
accumulation in the stratum corneum.
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Clobetasol propionate/in vitro skin penetration, Nanostructured lipid carriers/in vitro study, Proprionato de clobetasol/penetração cutânea in vitro, Carreadores lipídicos nanoestruturados/ estudo in vitro
Citação
SILVA, Luís Antônio Dantas et al.In skin penetration of clobetasol from lipid nanoparticles: drug extraction and quantitation in different skin layers. Brazilian Journal of Pharmaceutical Sciences, São Paulo, v. 48, n. 4, p. 811-817, Oct./Dec. 2012.