Monitorización terapéutica de la digoxinemia en pacientes con insuficiencia cardiaca: comparación entre dos métodos analíticos
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Data
2014
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Resumo
Objective: To compare two analytical techniques used in the determi-
nation of plasma digoxin (LC-MS/MS and immunoassay) and to verify
which one better answer the need of the clinical monitoring routine
of patients with cardiac heart failure.
Method: The clinical findings in 15 cardiac heart failure (CHF) outpa-
tients of the Cardiac Heart Service of the Goias Federal University Cli-
nical Hospital were investigated. Blood samples of the patients were
collected and analysed by Immunoassay and by Liquid Chromato-
graphy coupled to Mass Spectrometry (LC-MS/MS).
Results: The results of the statistic test (Student p = 0,05) showed a sig-
nificant difference between the analytical methods: immunoassay con-
centrations were higher than the concentrations determined by LC-
MS/MS. The explanation may be because immunoassay method
measures digoxin plus other metabolites and endogenous substances,
while the LC-MS/MS method measures only the digoxin molecule.
None of the patients, showed relevant clinical data suggestive of digi-
talis intoxication, even several drugs with potential interaction were
associated with treatment.
Conclusion: It was concluded, therefore, that LC-MS/MS me thod is
safer, more selective and specific than immunoassay, being an option
for therapeutic drug monitoring of digoxin, since the reference values
would be obtain for digoxinemia by LC-MS/MS.
Descrição
Palavras-chave
Digoxina, Inmunoensayo, Insuficiencia cardíaca, LC-MS/MS, Monitoreo plasmático, Digoxin, Cardiac heart failure, Immunoassay, Plasma concentrations, LC-MS/MS
Citação
BARROS, Isabel Cristina Medeiros et al. Monitorización terapéutica de la digoxinemia en pacientes con insuficiencia cardiaca: comparación entre dos métodos analíticos. Farmacia Hospitalaria, Toledo, v. 38, n. 1, p. 38-43, 2014.