Profile of antimicrobial resistance of bacteria isolated from cockroaches (Periplaneta americana) in a Brazilian health care institution
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2006-02
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Cockroaches carry microorganisms to sterilized materials, equipment and non-contaminated
food in hospitals and in homes. Hence, they are regarded as major microbial vectors. We isolated and
identified microorganisms from living cockroaches captured from a Brazilian health care institution
and we determined the antimicrobial susceptibility profile of the isolates. Following collection,
cockroaches were immobilized at 4°C for 10 to 20 minutes, immersed in 0.8% saline solution and
then homogenized. Next, the suspension was seeded in culture media: brain and heart agar infusion,
MacConkey agar, Naito agar, and they were incubated for up to 48 hours at 37ºC. Sabouraud agar
cultures were incubated at ambient temperature. The microbial colonies were analyzed with a
stereomicroscope to count and phenotypically identify the colony-forming units (cfu). The discdiffusion
method was used to determine the profile of susceptibility to antimicrobials. Among the 103
cockroaches analyzed, fungi were found in 97%, enterobacteria in 74.6% and coagulase-negative
staphylococci (CNS) in 25.40%. Among the enterobacteria, 96% were resistant to gentamicin, 84% to
ampicillin, 75.3% to caphalothin, 66.7% to ampicillin-sulbactam, 50% to aztreonam and 30% to
chloramphenicol. Among the CNS, 61% were resistant to oxacillin. We concluded that cockroaches
carry organisms associated with nosocomial infections and that are resistant to antimicrobials. This
fact contributes to the epidemiological chain, complicating therapeutics, and consequently, medical
costs are increased.
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Cockroach, Microorganism, Nosocomial infection, Antimicrobial resistance
Citação
PRADO, Marinésia Aparecida; GIR, Elucir; PEREIRA, Milca Severino; REIS, Cleomenes; PIMENTA, Fabiana Cristina. Profile antimicrobial resistance of bacteremia isoleted from cockroaches (periplaneta americana) in a Brazilian health care institution. The Brazilian Journal of Infectious Diseases, Salvador, v. 10, n. 1, p. 26-32, 2006.