Uma abordagem sobre métodos analíticos para determinação da atividade antioxidante em produtos naturais

Resumo

Antioxidants have been placing a highlighted position in the pharmaceutical industry, becoming the systematic research of these compounds important, for their ability to neutralize harmful agents in biological systems such as free radicals. In this work were evaluated some methodologies to determine the antioxidant capacity of some complex systems, as plant extracts, food, wine, among others. The detection of antioxidants was based on three categories of methods: electrochemical, colorimetric (DPPH e ABTS), and biological. Besides these, other instrumental techniques were pointed out, showing the versatility of the mechanisms that can be use to unveil the concentration of these substances. The HPLC (High Performance Liquid Chromatography) is a very useful tool in the identification and quantification of these compounds and can be coupled to the DPPH method. Each analytical method cited here has advantages and limitations, and the approach presented in this work ranges within these two parameters.

Descrição

Palavras-chave

Antioxidantes, CLAE, Radicais livres, DPPH, ABTS, Antioxidants, Free radicals, ABTS, DPPH, HPLC, Introdução

Citação

BORGES, Leonardo Luiz et al. Uma abordagem sobre métodos analíticos para determinação da atividade antioxidante em produtos naturais. Enciclopédia Biosfera, Goiânia v. 7, n. 12, p. 1-12, 2011.