Genetic structure and gene flow in Eugenia dysenterica DC in the Brasilian cerrado utilizing SSR markers
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Data
2003-12
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Resumo
The “cagaita tree” (Eugenia dysenterica) is a plant found widespread in the Brazilian Cerrado. Its fruit is used for
popular consumption and for industrial purposes. This study opens a new perspective for the generation of
population genetic data and parameters estimates for devising sound collection and conservation procedures for
Eugenia dysenterica. A battery of 356 primer pairs developed for Eucalyptus spp. was tested on the “cagaita tree”.
Only 10 primer pairs were found to be transferable between the two species. Using a polyacrilamide gel, an average
of 10.4 alleles per locus was detected, in a sample of 116 individuals from 10 natural “cagaita tree” populations.
Seven polymorphic loci allowed estimation of genetic parameters, including expected average heterozygosity He =
0,442, among population diversity, RST = 0,268 and gene flow Nm = 0,680. Results indicated a potential of SSR locus
transferability developed for Eucalyptus to other species of different genera, such as in the case of the “cagaita tree”.
The high genetic diversity among populations detected with SSR markers indicated that these markers are highly
sensitive to detect population structure. Estimated Nm values and the existence of private alleles indicated reduced
gene flow and consequently possible damage to the metapopulation structure.
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SSR, Myrtaceae, Cerrado, Tropical tree, Genetic diversity and transferability
Citação
ZUCCHI, Maria Imaculada et al. Genetic structure and gene flow in Eugenia dysenterica DC in the Brasilian Cerrado utilizing SSR markers. Genetics and Molecular Biology, Ribeirão Preto, v. 26, n.4, p. 449-457, Dec. 2003.