DNA extraction from human bone: comparison of magnetic bead and silica column techniques

Resumo

Objective: The present study aimed the comparison between the automated magnetic bead and silica column techniques for DNA extraction of human bones. Material and methods: Both techniques were performed on 25 human femur bones evaluating: I) the amount of extracted genetic material; II) the amount of amplified profiles; and III) the necessary time range to perform the techniques. Results: The automated magnetic bead technique recovered larger amount of DNA in 88% of the studied bone sample in comparison with the silica column technique. The automated magnetic bead technique also achieved a high level of amplifications (9/16 loci) in 68% of the sample, while the silica column technique reached equal level of amplifications only in 36% of the sample. The time range elapsed for performing the automated magnetic bead technique was approximately 3 hours for processing 12 samples, while the silica column technique performed the same samples in 81 hours. Conclusion: Based on that, the automated magnetic bead technique presented optimal outcomes and faster performance compared to the silica column technique, revealing a valuable tool for forensic DNA extraction.

Descrição

Palavras-chave

Magnetic bead, Partículas magnéticas, Coluna de sílica, Extração de DNA, Osso, Genética forense, Partículas magnéticas, Extração de DNA, Coluna de sílica, Genética forense, Osso

Citação

CANDIDO, Ian M. et al. A extraction from human bone: comparison of magnetic bead and silica column techniques. Robrac: revista odontologica do Brasil Central, Goiânia, v. 23, p. 155-158, 2014.