Microstructured liposome subunit vaccines reduce lung inflammationand bacterial load after Mycobacterium tuberculosis infection
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Data
2014
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Resumo
Tuberculosis is a disease affecting millions of people throughout the world. One of the mainproblems in controlling the disease is the low efficacy of the Bacillus Calmette–Guérin (BCG) vaccine inprotecting young adults. The development of new vaccines that induce a long-lasting immune responseor that stimulate the immunity induced by BCG may improve the control of tuberculosis.Methods: The use of microstructured liposomes containing HspX, with or without MPL or CpG DNAadjuvants, as vaccines for tuberculosis was evaluated. The HspX-specific humoral and cellular immuneresponses to the different vaccine formulations were compared.Results: All vaccines containing liposome microparticles and HspX were immunogenic. Vaccines formu-lated with CpG DNA and HspX induced the strongest humoral and cellular immune responses, mainlyby inducing interferon- and tumor necrosis factor- expression by both CD4+and CD8+T cells. HspXand MPL mainly induced CD8+T-cell activation and specific humoral responses. When evaluated theprotective efficacy of the formulations against Mycobacterium tuberculosis challenge, the microstruc-tured liposome containing L-HspX and L-HspX-CPG DNA reduced both lung inflammatory lesions andthe bacterial load.Conclusion: We have thus demonstrated, for the first time, the use of microstructured liposomes as anadjuvant and delivery system for a vaccine formulation against tuberculosis.
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Vaccine, Tuberculosis, Liposome, Adjuvants, Latent antigens, HspXa
Citação
TRENTINI, Monalisa Martins et al. Microstructured liposome subunit vaccines reduce lung inflammation and bacterial load after Mycobacterium tuberculosis infection. Vaccine, Ammsterdam, v. 32, n. 34, p. 4324-4332, 2014.