Immunization of mice with single PspA fragments induces antibodies capable of mediating complement deposition on different pneumococcal strains and cross-protection

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dc.creatorMoreno, Adriana Tonet
dc.creatorOliveira, Maria Leonor Sarno de
dc.creatorFerreira, Daniela Mulari
dc.creatorHo, Paulo Lee
dc.creatorDarrieux, Michelle
dc.creatorLeite, Luciana Cezar de Cerqueira
dc.creatorFerreira Júnior, Jorge Mario da Costa
dc.creatorPimenta, Fabiana Cristina
dc.creatorAndrade, Ana Lúcia Sampaio Sgambatti de
dc.creatorMiyaji, Eliane Namie
dc.date.accessioned2018-06-27T12:03:02Z
dc.date.available2018-06-27T12:03:02Z
dc.date.issued2010-03
dc.description.abstractPspA is an important candidate for a vaccine with serotype-independent immunity against pneumococcal infections. Based on sequence relatedness, PspA has been classified into three families comprising six clades. We have previously addressed the cross-reactivity of antibodies against PspA fragments containing the N-terminal and proline-rich regions of PspA from clades 1 to 5 (PspA1, PspA2, PspA3, PspA4, and PspA5) by Western blot analysis and reported that anti-PspA4 and anti-PspA5 were able to recognize pneumococci expressing PspA proteins from all of the clades analyzed. We have now analyzed the functional capacity of these antibodies to bind and to mediate complement deposition on intact bacteria in vitro. Our results show that both PspA4 and PspA5 elicit antibodies that are able to bind and to mediate complement deposition efficiently on pneumococcal strains bearing PspA proteins from clades 1 to 5. Moreover, mice immunized with PspA4 and PspA5 were protected against an intranasal lethal challenge with strains expressing PspA proteins from the two major families. PspA4 and PspA5 are thus able to induce antibodies with a high degree of cross-reactivity in vitro, which is reflected in cross-protection of mice. We have also analyzed the contribution of the nonproline (NonPro) block within the conserved proline-rich region to the reactivity of anti-PspA antibodies, and the results indicate that N-terminal -helical region, the blocks of proline repeats, and the NonPro region can influence the degree of cross-reactivity of antibodies to PspA.pt_BR
dc.identifier.citationMoreno, Adriana T. et. al. Immunization of mice with single PspA fragments induces antibodies capable of mediating complement deposition on different pneumococcal strains and cross-protection. Clinical and Vaccine Immunology, Washington, v. 17, n. 3, p. 439-446, Mar. 2010.pt_BR
dc.identifier.doi10.1128/CVI.00430-09
dc.identifier.issn1556-6811
dc.identifier.issne-1556-679X
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/15299
dc.language.isoengpt_BR
dc.publisher.countryEstados unidospt_BR
dc.publisher.departmentInstituto de Patologia Tropical e Saúde Pública - IPTSP (RG)pt_BR
dc.rightsAcesso Abertopt_BR
dc.titleImmunization of mice with single PspA fragments induces antibodies capable of mediating complement deposition on different pneumococcal strains and cross-protectionpt_BR
dc.typeArtigopt_BR

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