Standardization of in-house polymerase chain reaction for the identification of Mycobacterium tuberculosis at the reference tropical disease hospital in the state of Goiás, Brazil

Página do item simplificado
dc.creatorRodrigues, Márcia Alves Vasconcelos
dc.creatorSerafini, Álvaro Bisol
dc.creatorPereira, Marieta de Souza
dc.creatorSilva, Thathiane Dias da
dc.creatorRabahi, Marcelo Fouad
dc.creatorAlves, Suely Lemes de
dc.creatorKipnis, André
dc.date.accessioned2018-12-13T12:54:24Z
dc.date.available2018-12-13T12:54:24Z
dc.date.issued2004
dc.description.abstractThis study compares smear, growth in Lowenstein-Jensen medium, and in-house polymerase chain reaction (PCR) techniques for the detection of Mycobacterium tuberculosis. A total of 72 specimens from 72 patients with clinical symptoms of tuberculosis, including 70 sputum and two bronchial aspirate samples, were tested in parallel by smear, culture, and in-house PCR techniques. From these, 48 (66.6%) were negative by the 3 methods, 2 (2.8%) were smear positive and negative by culture and in-house PCR, 11 (15.3%) were both smear and culture negative, and in-house PCR positive, 7 (9.7%) were positive by the 3 methods, 2 (2.8%) were positive by smear and culture, and negative by PCR, 2 (2.8%) were positive by culture and PCR, but smear negative. After the resolution of discrepancies in PCR results, the sensitivity and specificity for in-house PCR technique to M. tuberculosis relative to the culture, were 81.8% and 81.9%, respectively. These results confirm that this method, in-house PCR, may be a sensitive and specific technique for M. tuberculosis detection, occurring in both positive and negative smear and negative cultures.pt_BR
dc.identifier.citationRODRIGUES, Márcia Alves Vasconcelos; SERAFINI, Álvaro Bisol; PEREIRA, Marieta de Souza; SILVA, Thathiane Dias da; RABAHI, Marcelo Fouad; ALVES, Suely Lemes de; KIPNIS, André. Standardization of in-house polymerase chain reaction for the identification of Mycobacterium tuberculosis at the reference tropical disease hospital in the state of Goiás, Brazil. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 99, n. 4, p. 415-419, 2004.pt_BR
dc.identifier.doi10.1590/S0074-02762004000400012
dc.identifier.issn0074-0276
dc.identifier.issne- 1678-8060
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/16576
dc.language.isoengpt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentInstituto de Patologia Tropical e Saúde Pública - IPTSP (RG)pt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectSmearpt_BR
dc.subjectMycobacterium tuberculosispt_BR
dc.subjectPolymerase chain reactionpt_BR
dc.subjectTuberculosispt_BR
dc.subjectCulturept_BR
dc.titleStandardization of in-house polymerase chain reaction for the identification of Mycobacterium tuberculosis at the reference tropical disease hospital in the state of Goiás, Brazilpt_BR
dc.typeArtigopt_BR

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
Artigo - Márcia Alves Vasconcelos Rodrigues - 2004.pdf
Tamanho:
47.08 KB
Formato:
Adobe Portable Document Format
Baixar
Licença do Pacote
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Baixar

Coleções

IPTSP - Artigos publicados em periódicos