Biofilms of black tooth stains: pcr analysis reveals presence of streptococcus mutans

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dc.creatorCosta, Marilia Teixeira
dc.creatorDorta, Miriam Cristina Leandro
dc.creatorDias, Fátima Ribeiro
dc.creatorPimenta, Fabiana Cristina
dc.date.accessioned2018-07-19T15:47:04Z
dc.date.available2018-07-19T15:47:04Z
dc.date.issued2012
dc.description.abstractThis study investigated the presence of the black-pigmented bacteria Prevotella nigrescens and Prevotella intermedia, the non-black- pigmented bacteria Actinomyces spp and particularly the cariogenic pathogen Streptococcus mutans in the dental biofilms of patients with or without black extrinsic tooth stains, using the multiplex polymerase chain reaction (PCR) technique. Analysis of the dental biofilms of patients with (n=26) or without (n=26) black tooth stains was performed using duplex PCR for the 16S ribosomal RNA gene (P. nigrescens, P. intermedia, Actinomyces spp) and glucosyltransferase-I gene for S. mutans. P. nigrescens and S. mutans were the most frequent bacteria detected in both groups. The least frequently detected were P. intermedia and Actinomyces spp. The similar bacterial composition of dental biofilms of black tooth stains and healthy tooth surfaces indicates that black tooth stains are not free of cariogenic bacteria.pt_BR
dc.identifier.citationCOSTA, Marília Teixeira ; DORTA, Miriam Leandro ; RIBEIRO-DIAS, Fátima; PIMENTA, Fabiana Cristina. Biofilms of black tooth stains: pcr analysis reveals presence of streptococcus mutans. Brazilian Dental Journal, Ribeirão Preto, v. 23, n. 5, p. 555-558, Sept./Oct. 2012.pt_BR
dc.identifier.doi10.1590/S0103-64402012000500014
dc.identifier.issn0103-6440
dc.identifier.issne- 1806-4760
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/15427
dc.language.isoengpt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentInstituto de Patologia Tropical e Saúde Pública - IPTSP (RG)pt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectBlack tooth stainpt_BR
dc.subjectBlack-pigmented bacteriapt_BR
dc.subjectPCRpt_BR
dc.subjectDdental cariespt_BR
dc.subjectMutans streptococcipt_BR
dc.titleBiofilms of black tooth stains: pcr analysis reveals presence of streptococcus mutanspt_BR
dc.typeArtigopt_BR

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