Development of microsatellite markers for Hoplias malabaricus (Erythrinidae)

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dc.creatorGondim, Sara Giselle de Cassia Alexandre
dc.creatorResende, Lucileide Vilela
dc.creatorBrondani, Rosana Pereira Vianello
dc.creatorCollevatti, Rosane Garcia
dc.creatorSilva Júnior, Nelson Jorge da
dc.creatorPereira, Rodrigo Roncato
dc.date.accessioned2017-07-10T15:02:39Z
dc.date.available2017-07-10T15:02:39Z
dc.date.issued2010-08
dc.description.abstractWe identified 14 microsatellite loci for the wolf fish, Hoplias malabaricus (Erythrinidae), from a genomic shotgun library. Twenty-five primers were designed, and 48 individuals of H. malabaricus from four localities of northwest Goiás, in central Brazil, were genotyped to characterize the polymorphism at each locus. Fourteen primers amplified clearly interpretable products using a single PCR protocol; six loci were polymorphic, but with a low number of alleles per locus (2 or 3). Expected heterozygosities for polymorphic loci ranged from 0.136 to 0.505. Combined paternity exclusion probability (0.638) was low and combined genetic identity (0.056) was high in studies of parentage. The low polymorphism may be due to the small microsatellite size and the large size of the motifs.pt_BR
dc.identifier.citationGONDIM, S. G. C. A.; RESENDE, L. V.; BRONDANI, R. P. V.; COLLEVATTI, R. G.; SILVA JÚNIOR, N. J.; PEREIRA, R. R.; TELLES, M. P. C. Development of microsatellite markers for Hoplias malabaricus (Erythrinidae). Genetics and Molecular Research, Ribeirão Preto, v. 9, n. 3, p. 1513-1517, Aug. 2010.pt_BR
dc.identifier.doi10.4238/vol9-3gmr877
dc.identifier.issne- 1676-5680
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/12045
dc.language.isoengpt_BR
dc.publisherFundação de Pesquisas Científicas de Ribeirão Pretopt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentInstituto de Ciências Biológicas - ICB (RG)pt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectCerradopt_BR
dc.subjectErythrinidaept_BR
dc.subjectMicrosatellitept_BR
dc.subjectShotgun librarypt_BR
dc.titleDevelopment of microsatellite markers for Hoplias malabaricus (Erythrinidae)pt_BR
dc.typeArtigopt_BR

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