Distinct chitinases are expressed during various growth phases of the human pathogen Paracoccidioides brasiliensis

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dc.creatorSantana, Lidiane Aparecida da Penha
dc.creatorVainstein, Marilene Henning
dc.creatorTomazett, Patrícia Kott
dc.creatorSilva, Ludier Kesser Santos
dc.creatorGóes, Alfredo Miranda
dc.creatorSchrank, Augusto
dc.creatorSoares, Célia Maria de Almeida
dc.creatorPereira, Maristela
dc.date.accessioned2018-07-05T11:19:25Z
dc.date.available2018-07-05T11:19:25Z
dc.date.issued2012-05
dc.description.abstractThe aim of this work was the partial purification and subsequent evaluation of chitinase expression during the various growth phases of Paracoccidioides brasiliensis. Initially, PbCTS1r was expressed as a recombinant pro- tein and displayed enzymatic activity against 4-MU-[N-acetylglucosamine (GlcNAc)] 3 and 4-MU-(GlcNAc) 2 . Two proteins, 45 kDa and 39 kDa in size, were partially purified from P. brasiliensis yeast crude extract using cation- exchange chromatography coupled with HPLC and were characterised as PbCTS1 and PbCTS2, respectively. Anti- PbCTS1r antibody recognised two proteins in the crude extracts of yeast and the transitional stage between mycelial and yeast phases. In crude extracts of mycelium, only the 45 kDa protein was detected. However, quantitative real- time polymerase chain reaction led to the detection of small quantities of Pbcts2 transcript in the mycelial phase. In the yeast cell wall extract, only the 39 kDa protein was detected. Moreover, both proteins were secreted by the yeast parasitic phase, suggesting that these proteins participate in the modulation of the fungal environment. Phylogenetic analysis of the predicted PbCTS1 and PbCTS2 proteins indicated that they code for distinct chitinases in P. brasilien- sis. During evolution, P. brasiliensis could have acquired the paralogues Pbcts1 and Pbcts2 for growth and survival in diverse environments in both saprophytic and parasitic phases.pt_BR
dc.identifier.citationSANTANA, Lidiane Aparecida da Penha; VAINSTEIN, Marilene Henning; TOMAZETT, Patrícia Kott; SANTOS-SILVA, Ludier Kesser; GÓES, Alfredo Miranda; SCHRANK, Augusto; SOARES, Célia Maria de Almeida; PEREIRA, Maristela. Distinct chitinases are expressed during various growth phases of the human pathogen Paracoccidioides brasiliensis. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 107, n. 3, p. 310-316, May 2012.pt_BR
dc.identifier.doi10.1590/S0074-02762012000300004
dc.identifier.issn0074-0276
dc.identifier.issne- 1678-8060
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/15343
dc.language.isoengpt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentInstituto de Ciências Biológicas - ICB (RG)pt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectParacoccidioidespt_BR
dc.subjectCell wallpt_BR
dc.subjectChitinasept_BR
dc.subjectChromatographypt_BR
dc.titleDistinct chitinases are expressed during various growth phases of the human pathogen Paracoccidioides brasiliensispt_BR
dc.typeArtigopt_BR

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