Antioxidant effect of 4-nerolidylcatechol and α-tocopherol in erythrocyte ghost membranes and phospholipid bilayers

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dc.creatorFernandes, Kelly de Souza
dc.creatorSilva, Adolfo Henrique de Moraes
dc.creatorMendanha Neto, Sebastião Antônio
dc.creatorRezende, Kennia Rocha
dc.creatorAlonso, Antonio
dc.date.accessioned2018-06-14T11:43:12Z
dc.date.available2018-06-14T11:43:12Z
dc.date.issued2013
dc.description.abstract4-Nerolidylcatechol (4-NC) is found in Pothomorphe umbellata root extracts and is reported to have a topical protective effect against UVB radiation-induced skin damage, toxicity in melanoma cell lines, and antimalarial activity. We report a comparative study of the antioxidant activity of 4-NC and a-tocopherol against lipid peroxidation initiated by two free radical-generating systems: 2,29-azobis(2-aminopropane) hydrochloride (AAPH) and FeSO4/H2O2, in red blood cell ghost membranes and in egg phosphatidylcholine (PC) vesicles. Lipid peroxidation was monitored by membrane fluidity changes assessed by electron paramagnetic resonance spectroscopy of a spin-labeled lipid and by the formation of thiobarbituric acid-reactive substances. When lipoperoxidation was initiated by the hydroxyl radical in erythrocyte ghost membranes, both 4-NC and a-tocopherol acted in a very efficient manner. However, lower activities were observed when lipoperoxidation was initiated by the peroxyl radical; and, in this case, the protective effect of a-tocopherol was lower than that of 4-NC. In egg PC vesicles, malondialdehyde formation indicated that 4-NC was effective against lipoperoxidation initiated by both AAPH and FeSO4/H2O2, whereas atocopherol was less efficient in protecting against lipoperoxidation by AAPH, and behaved as a pro-oxidant for FeSO4/H2O2. The DPPH (2,2-diphenyl-1-picrylhydrazyl) free-radical assay indicated that two free radicals were scavenged per 4-NC molecule, and one free radical was scavenged per a-tocopherol molecule. These data provide new insights into the antioxidant capacity of 4-NC, which may have therapeutic applications for formulations designed to protect the skin from sunlight irradiation.pt_BR
dc.identifier.citationFERNANDES, K. S.; SILVA, A. H. M.; MENDANHA, S. A.; REZENDE, K. R.; ALONSO, A. Antioxidant effect of 4-nerolidylcatechol and α-tocopherol in erythrocyte ghost membranes and phospholipid bilayers. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 46, n. 9, p. 780-788, Sept. 2013.pt_BR
dc.identifier.doi10.1590/1414-431X20132940
dc.identifier.issne- 1414-431X
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/15257
dc.language.isoengpt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentInstituto de Física - IF (RG)pt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectElectron paramagnetic resonancept_BR
dc.subject4-Nerolidylcatecholpt_BR
dc.subjectSpin labelpt_BR
dc.subjectLipid peroxidationpt_BR
dc.subjectDPPHpt_BR
dc.titleAntioxidant effect of 4-nerolidylcatechol and α-tocopherol in erythrocyte ghost membranes and phospholipid bilayerspt_BR
dc.typeArtigopt_BR

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