Cell death in amastigote forms of Leishmania amazonensis induced by parthenolide

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dc.creatorTiuman, Tatiana Shioji
dc.creatorNakamura, Tânia Ueda
dc.creatorAlonso, Antonio
dc.creatorNakamura, Celso Vataru
dc.date.accessioned2018-06-14T11:53:02Z
dc.date.available2018-06-14T11:53:02Z
dc.date.issued2014
dc.description.abstractBackground: Leishmania amazonensis infection results in diverse clinical manifestations: cutaneous, mucocutaneous or visceral leishmaniasis. The arsenal of drugs available for treating Leishmania infections is limited. Therefore, new, effective, and less toxic leishmaniasis treatments are still needed. We verified cell death in amastigote forms of Leishmania amazonensis induced by the sesquiterpene lactone parthenolide. Results: The tested compound was able to concentration-dependently affect axenic and intracellular amastigotes, with IC50 values of 1.3 μM and 2.9 μM, respectively after 72 h incubation. No genotoxic effects were observed in a micronucleus test in mice. Parthenolide induced morphological and ultrastructural changes in axenic amastigotes, including a loss of membrane integrity, swelling of the mitochondrion, cytoplasmic vacuoles, and intense exocytic activity in the region of the flagellar pocket. These results led us to investigate the occurrence of autophagic vacuoles with monodansylcadaverine and the integrity of the plasma membrane and mitochondrial membrane potential using flow cytometry. In all of the tests, parthenolide had positive results. Conclusions: Our results indicate that the antileishmanial action of parthenolide is associated with autophagic vacuole appearance, a reduction of fluidity, a loss of membrane integrity, and mitochondrial dysfunction. Considering the limited repertoire of existing antileishmanial compounds, the products derived from medicinal plants has been one the greatest advances to help develop new chemotherapeutic approaches.pt_BR
dc.identifier.citationTIUMAN, Tatiana Shioji; UEDA-NAKAMURA, Tânia; ALONSO, Antonio; NAKAMURA, Celso Vataru. Cell death in amastigote forms of Leishmania amazonensis induced by parthenolide. BMC Microbiology, London, v. 14, n. 152, p. 1-12, 2014.pt_BR
dc.identifier.doi10.1186/1471-2180-14-152
dc.identifier.issne- 1471-2180
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/15259
dc.language.isoengpt_BR
dc.publisher.countryOutrospt_BR
dc.publisher.departmentInstituto de Física - IF (RG)pt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectLeishmania amazonensispt_BR
dc.subjectAmastigotespt_BR
dc.subjectParthenolidept_BR
dc.subjectCell deathpt_BR
dc.subjectAutophagypt_BR
dc.titleCell death in amastigote forms of Leishmania amazonensis induced by parthenolidept_BR
dc.typeArtigopt_BR

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