DNA damage in peripheral blood lymphocytes and association with polymorphisms in the promoter region of the CYP2E1 gene in alcoholics from Central Brazil

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dc.creatorRamos, Jheneffer Sonara Aguiar
dc.creatorAlves, Alessandro Arruda
dc.creatorLopes, Mariana Paiva
dc.creatorPedroso, Thays Millena Alves
dc.creatorFelício, Leandro Prado
dc.creatorCarvalho, Wanessa Fernandes
dc.creatorFranco, Fernanda Craveiro
dc.creatorMelo, Caroline Oliveira de Araújo
dc.creatorGonçalves, Macks Wendhell
dc.creatorSoares, Thannya Nascimento
dc.date.accessioned2023-08-31T14:46:29Z
dc.date.available2023-08-31T14:46:29Z
dc.date.issued2016
dc.description.abstractDNA damage caused by the accumulation of bio-products generated in the biotransformation of ethanol to acetaldehyde mediated by the CYP2E1 enzyme has been studied. To evaluate DNA damage in peripheral blood lymphocytes and the possible association with polymorphisms in the promoter region of the CYP2E1 gene, we performed a case-control study including 75 alcoholics and 59 individuals who consume alcohol socially. Alcoholics were previously diagnosed by the Psychosocial Care Center – Alcohol and Drugs (CAPS A/D) in the city of Goiania, Goias state, Central Brazil. DNA damage was evaluated by comet assay. The analysis of the rs3813867, rs2031920, and rs2031921 polymorphisms in the promoter region of CYP2E1 gene was performed by Sanger sequencing. Men older than 35 years old were the most common alcoholics. We found increased DNA damage in the case group, compared to the control group (p < 0.001). Alcoholics who were heterozygous in the rs3813867, rs2031920, and rs2031921 polymorphisms showed higher DNA damage (tail length and olive tail moment), compared to individuals with the homozygous non-mutated allele. Previous studies have shown that polymorphisms in the promoter region of the CYP2E1 gene could cause higher CYP2E1 transcriptional activity, increasing enzyme activity compared with nondrinkers, indicating that the presence of the mutated allele (heterozygous or homozygous) may be associated with higher alcohol metabolic rates and therefore show increased acetaldehyde levels after alcohol consumption, which then can exert its carcinogenic effect.pt_BR
dc.identifier.citationRAMOS, Jheneffer Sonara Aguiar et al. DNA damage in peripheral blood lymphocytes and association with polymorphisms in the promoter region of the CYP2E1 gene in alcoholics from Central Brazil. Alcohol, Amsterdan, v. 57, p. 35-39, 2016. DOI: 10.1016/j.alcohol.2016.08.007. Disponível em: https://www.sciencedirect.com/science/article/pii/S0741832916301550. Acesso em 30 ago. 2023.pt_BR
dc.identifier.doi10.1016/j.alcohol.2016.08.007
dc.identifier.issn 0741-8329
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0741832916301550
dc.language.isoengpt_BR
dc.publisher.countryHolandapt_BR
dc.publisher.departmentInstituto de Ciências Biológicas - ICB (RMG)pt_BR
dc.rightsAcesso Restritopt_BR
dc.subjectCYP2E1pt_BR
dc.subjectDNA damagept_BR
dc.subjectEthanolpt_BR
dc.subjectMetabolismpt_BR
dc.subjectPolymorphismpt_BR
dc.titleDNA damage in peripheral blood lymphocytes and association with polymorphisms in the promoter region of the CYP2E1 gene in alcoholics from Central Brazilpt_BR
dc.typeArtigopt_BR

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