The Trypanosoma cruzi Satellite DNA OligoC-TesT and Trypanosoma cruzi Kinetoplast DNA OligoC-TesT for diagnosis of Chagas disease: a multi-cohort comparative evaluation study

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dc.creatorDe Winne, Koen
dc.creatorBuscher, Philippe
dc.creatorOstermayer, Alejandro Luquetti
dc.creatorTavares, Suelene Brito do Nascimento
dc.creatorOliveira, Rodrigo A.
dc.creatorSolari, Aldo
dc.creatorZulantay, Ines
dc.creatorApt, Werner
dc.creatorDiosque, Patricio
dc.creatorRumi, Mercedes Monje
dc.creatorGironès, Nuria
dc.creatorFresno, Manuel
dc.creatorLopez-Velez, Rogelio
dc.creatorPerez-Molina, José Antônio
dc.creatorMonge-Maillo, Begoña
dc.creatorGarcia, Lineth
dc.creatorDeborggraeve, Stijn
dc.date.accessioned2018-03-20T10:29:48Z
dc.date.available2018-03-20T10:29:48Z
dc.date.issued2014-01
dc.description.resumoBackground: The Trypanosoma cruzi satellite DNA (satDNA) OligoC-TesT is a standardised PCR format for diagnosis of Chagas disease. The sensitivity of the test is lower for discrete typing unit (DTU) TcI than for TcII-VI and the test has not been evaluated in chronic Chagas disease patients. Methodology/Principal Findings: We developed a new prototype of the OligoC-TesT based on kinetoplast DNA (kDNA) detection. We evaluated the satDNA and kDNA OligoC-TesTs in a multi-cohort study with 187 chronic Chagas patients and 88 healthy endemic controls recruited in Argentina, Chile and Spain and 26 diseased non-endemic controls from D.R. Congo and Sudan. All specimens were tested in duplicate. The overall specificity in the controls was 99.1% (95% CI 95.2%–99.8%) for the satDNA OligoC-TesT and 97.4% (95% CI 92.6%–99.1%) for the kDNA OligoC-TesT. The overall sensitivity in the patients was 67.9% (95% CI 60.9%–74.2%) for the satDNA OligoC-TesT and 79.1% (95% CI 72.8%–84.4%) for the kDNA OligoC-Test. Conclusions/Significance: Specificities of the two T. cruzi OligoC-TesT prototypes are high on non-endemic and endemic controls. Sensitivities are moderate but significantly (p = 0.0004) higher for the kDNA OligoC-TesT compared to the satDNA OligoC-TesT.pt_BR
dc.identifier.citationDE WINNE, Koen; BÜSCHER, Philippe; LUQUETTI, Alejandro O.; TAVARES, Suelene B. N.; OLIVEIRA, Rodrigo A.; SOLARI, Aldo; ZULANTAY, Ines; APT, Werner; DIOSQUE, Patricio; RUMI, Mercedes Monje; GIRONÈS, Nuria; FRESNO, Manuel; LOPEZ-VELEZ, Rogelio; PEREZ-MOLINA, José A.; MONGE-MAILLO, Begoña; GARCIA, Lineth; DEBORGGRAEVE, Stijn. The Trypanosoma cruzi Satellite DNA OligoC-TesT and Trypanosoma cruzi Kinetoplast DNA OligoC-TesT for diagnosis of Chagas disease: a multi-cohort comparative evaluation study. PLoS Neglected Tropical Diseases, San Francisco, v. 8, n. 1, p. e2633, Jan. 2014.pt_BR
dc.identifier.doi10.1371/ journal.pntd.0002633
dc.identifier.issn1935-2727
dc.identifier.issne- 1935-2735
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/14060
dc.language.isoengpt_BR
dc.publisher.countryEstados unidospt_BR
dc.publisher.departmentInstituto de Patologia Tropical e Saúde Pública - IPTSP (RG)pt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectTrypanosoma cruzipt_BR
dc.subjectChagas diseasept_BR
dc.subjectDiagnosis of Chagas diseasept_BR
dc.titleThe Trypanosoma cruzi Satellite DNA OligoC-TesT and Trypanosoma cruzi Kinetoplast DNA OligoC-TesT for diagnosis of Chagas disease: a multi-cohort comparative evaluation studypt_BR
dc.typeArtigopt_BR

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