Heterologous expression, purification and immunological reactivity of a recombinant HSP60 from Paracoccidioides brasiliensis

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dc.creatorCunha, Daniela A.
dc.creatorOliveira, Rosely Maria Zancopé
dc.creatorFelipe, Maria Sueli Soares
dc.creatorFurlaneto, Silvia Maria Salem Izacc
dc.creatorDeepe Junior, George S.
dc.creatorSoares, Célia Maria de Almeida
dc.date.accessioned2018-07-16T12:03:42Z
dc.date.available2018-07-16T12:03:42Z
dc.date.issued2002-03
dc.description.abstractThe complete coding cDNA of HSP60 from Paracoccidioides brasiliensis was overexpressed in an Escherichia coli host to produce high levels of recombinant protein. The protein was purified by affinity chromatography. A total of 169 human serum samples were tested for reactivity by Western blot analysis with the purified HSP60 recombinant protein. Immunoblots indicated that the recombinant P. brasiliensis HSP60 was recognized by antibodies in 72 of 75 sera from paracoccidioidomycosis patients. No cross-reactivity was detected with individual sera from patients with aspergillosis, sporotrichosis, cryptococcosis, and tuberculosis. Reactivity to HSP60 was observed in sera from 9.52% of control healthy individuals and 11.5% of patients with histoplas- mosis. The high sensitivity and specificity (97.3 and 92.5%, respectively) for HSP60 suggested that the recombinant protein can be used singly or in association with other recombinant antigens to detect antibody responses in P. brasiliensis-infected patients.pt_BR
dc.identifier.citationCUNHA, Daniela A.; ZANCOPÉ-OLIVEIRA, Roseli M.; FELIPE, M. Sueli S.; SALEM-IZACC, Silvia M.; DEEPE JR, George S ; SOARES, Célia M. A. Heterologous expression, purification and immunological reactivity of a recombinant HSP60 from Paracoccidioides brasiliensis. Clinical and Diagnostic Laboratory Immunology, Washington, v. 9, n. 2, p. 374-377, Mar. 2002.pt_BR
dc.identifier.doi10.1128/CDLI.9.2.374–377.2002
dc.identifier.issn1071-412X
dc.identifier.issne- 1098-6588
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/15406
dc.language.isoengpt_BR
dc.publisher.countryEstados unidospt_BR
dc.publisher.departmentInstituto de Ciências Biológicas - ICB (RG)pt_BR
dc.rightsAcesso Abertopt_BR
dc.titleHeterologous expression, purification and immunological reactivity of a recombinant HSP60 from Paracoccidioides brasiliensispt_BR
dc.typeArtigopt_BR

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