Accuracy in clinical examinations for the diagnosis of vulvovaginitis by Candida ssp. and In vitro susceptibility to the main antifungals

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dc.creatorSantos, Andressa Santana
dc.creatorZara, Ana Laura de Sene Amâncio
dc.creatorAtaídes, Fábio Silvestre
dc.creatorGomes, Elisangela Christhianne Barbosa da Silva
dc.creatorFreitas, Vivianny Aparecida Queiroz
dc.creatorCosta, Carolina Rodrigues
dc.creatorSilva, Thaísa Cristina
dc.creatorSilva, Maria do Rosário Rodrigues
dc.date.accessioned2022-03-17T13:12:13Z
dc.date.available2022-03-17T13:12:13Z
dc.date.issued2020-09-29
dc.description.abstractVulvovaginal candidiasis (VVC) is a common infection. This work aims to determine the positive predictive value (PPV) of the clinical diagnosis of VVC and to characterize Candida species isolated from the vaginal mucosa. This cross-sectional study was conducted from February 2016 to February 2017 at the Gynecology and Obstetrics Outpatient Clinic of the Hospital das Clínicas, in Goiânia, Goiás State, Brazil. The study included samples of vaginal secretion from 55 women who complained of vaginal discharge and itching as their main symptoms. The PPV of the clinical diagnosis of VVC was estimated in comparison to the laboratory culture method. The phenotypic methods and molecular tests were performed to identify Candida spp. In vitro susceptibility of Candida spp. isolates to fluconazole, itraconazole, clotrimazole, nystatin, and amphotericin B was determined using the broth microdilution assay. Yeast growth using the enzymes protease, phospholipase, and hemolysin was carried out in media containing respectively bovine albumin, egg yolk, and sheep erythrocytes. A PPV of 61.8% (34/55) was determined. Among the 55 vulvovaginal samples collected, we identified 36 isolates in which C. albicans was the most common species. High resistance to fluconazole and low minimal inhibitory concentration (MIC) values for clotrimazole, nystatin and amphotericin B were observed. All isolates were proteinase and hemolysin producers, while seven strains were phospholipase negative. The clinical diagnosis of VVC presented a moderate PPV, which meant that cultures had to be conducted in the laboratory to confirm infection. The high resistance to fluconazole and itraconazole indicated the importance of the in vitro susceptibility test.pt_BR
dc.identifier.citationSANTOS, Andressa Santana; ZARA, Ana Laura Sene Amâncio; ATAÍDES, Fábio Silvestre;SILVA, Elisangela Gomes da; FREITAS,Vivianny Aparecida Queiroz; COSTA,Carolina Rodrigues; SILVA, Thaísa Cristina; DO SILVA, Maria do Rosário Rodrigues. Accuracy in clinical examinations for the diagnosis of vulvovaginitis by Candida ssp. and In vitro susceptibility to the main antifungals. Revista de Patologia Tropical, Goiânia, v. 49, n. 3, july/Sept. 2020. Disponível em: https://www.revistas.ufg.br/iptsp/article/view/64233.pt_BR
dc.identifier.doi10.5216/rpt.v49i3.64233
dc.identifier.issn1980-8178
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/20400
dc.language.isoengpt_BR
dc.publisherUniversidade Federal de Goiáspt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentInstituto de Patologia Tropical e Saúde Pública - IPTSP (RG)pt_BR
dc.publisher.initialsUFGpt_BR
dc.rightsAcesso Abertopt_BR
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectVulvovaginal candidiasispt_BR
dc.subjectAntifungal susceptibilitypt_BR
dc.subjectEnzymatic activitypt_BR
dc.titleAccuracy in clinical examinations for the diagnosis of vulvovaginitis by Candida ssp. and In vitro susceptibility to the main antifungalspt_BR
dc.typeArtigopt_BR

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