Detection from calicivirus from fecal samples from children with acute gastroenteritis in the West Central Region of Brazil

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dc.creatorBorges, Ana Maria Tavares
dc.creatorTeixeira, Jose Marcus Socrates
dc.creatorCosta, Paulo Sérgio Sucasas da
dc.creatorGiugliano, Loreny Gimenes
dc.creatorFiaccadori, Fabíola Souza
dc.creatorFranco, Rachel de Carvalho e
dc.creatorBrito, Wilia Marta Elsner Diederichsen de
dc.creatorLeite, José Paulo Gagliardi
dc.creatorCardoso, Divina das Dores de Paula
dc.date.accessioned2018-07-19T12:02:53Z
dc.date.available2018-07-19T12:02:53Z
dc.date.issued2006-11
dc.description.abstractThe objective of this study was to describe the circulation of caliciviruses in the West Central region of Brazil and its correlation with children’s gender and age, as well as with the year and months of the sample collection. Reverse transcriptase-polymerase chain reaction was performed to detect the human calicivirus genome in 1006 fecal samples that were collected in Goiânia (n = 696) and Brasília (n = 310). Viral RNA was detected in 8.6% of the samples. No significant difference in viral prevalence was found regarding gender, age or year of the sample. However, it was observed that in Goiânia, there is a higher incidence of caliciviruses from September to March. The analysis employing three primer pairs demonstrated that the Ni/E3 or JV12/13 primer pairs, which detect norovirus (NoV), detected 41 positive samples while the 289/290 primer pair, which detects NoV or sapovirus, detected the remaining 46 samples. Calicivirus circulates in the West Central region of Brazil and for better detection of this virus it is important to use more than one primer pair. Also, we conclude that the seasonality presented by this virus is related to higher humidity in the period.pt_BR
dc.identifier.citationBORGES, Ana Maria Tavares; TEIXEIRA, José Marcus Sócrates; COSTA, Paulo Sérgio Sucasas da; GIULIANO, Loreny Gimenes; FIACCADORI, Fabíola Souza; FRANCO, Rachel de Carvalho e; BRITO, Wília Marta Elsner Diederichsen de; LEITE, José Paulo Gagliardi; CARDOSO, Divina das Dôres de Paula. Detection from calicivirus from fecal samples from children with acute gastroenteritis in the West Central Region of Brazil. Memórias do Instituto Oswaldo Cruz, Brasil, Rio de Janeiro, v. 101, n. 7, p. 721-724, Nov. 2006.pt_BR
dc.identifier.doi10.1590/S0074-02762006000700003
dc.identifier.issn0074-0276
dc.identifier.issne- 1678-8060
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/15426
dc.language.isoengpt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentInstituto de Patologia Tropical e Saúde Pública - IPTSP (RG)pt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectCaliciviruspt_BR
dc.subjectBrazilpt_BR
dc.subjectAcute gastroenteritispt_BR
dc.subjectReverse transcriptasept_BR
dc.subjectPolymerase chain reactionpt_BR
dc.titleDetection from calicivirus from fecal samples from children with acute gastroenteritis in the West Central Region of Brazilpt_BR
dc.typeArtigopt_BR

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