Sensibilidade da PCR na amplificação do DNA bovino em diluição seriada e mistura de amostra macho e fêmea

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dc.creatorCruz, Alex Silva da
dc.creatorSilva, Danilo Conrado
dc.creatorCosta, Emília Oliveira Alves
dc.creatorSilva, Daniela de Melo e
dc.creatorAbreu, D. A.
dc.creatorCruz, Aparecido Divino da
dc.date.accessioned2018-08-01T15:48:36Z
dc.date.available2018-08-01T15:48:36Z
dc.date.issued2011
dc.description.abstractA mixture of bovine DNA from a male and a female Jersey (Bos taurus taurus) bred in different proportions was used to determine the sensitivity of PCR to amplify and discriminate the bovine DNA samples. Samples were obtained from the peripheral blood of a bull and a heifer and DNA was isolated using a commercial kit for extraction and purification of nucleic acids. Two primers sets were designed to flank genomic regions: one autosomal and one Y-specific. DNA samples were diluted in water to a final concentration of 4x10-14 ng. The results showed positive amplification of the samples diluted to a concentration of 4x10-10ng and 4x10-4ng for the autosomal and Y-specific regions, respectively. PCR was able to discriminate the male DNA in a mixture of 99:1 (DNA ♀: DNA ♂) heifer to bull ratio. Therefore, the PCR was successful in amplifying the bovine genome in samples containing low concentrations of DNA. Thus, PCR can be used as a sensitive and efficient tool to determine the sex of the fetus in pregnant cows, helping to promote correct and efficient animal management, sex selection, and breeding in commercial herds.pt_BR
dc.identifier.citationCRUZ, A. S.; SILVA, D. C.; COSTA, E. O. A.; SILVA, D. M.; ABREU, D. A.; CRUZ, A. D. Sensibilidade da PCR na amplificação do DNA bovino em diluição seriada e mistura de amostra macho e fêmea. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 63, n. 4, p. 1012-1015, 2011.pt_BR
dc.identifier.doi10.1590/S0102-09352011000400030
dc.identifier.issne- 1678-4162
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/15517
dc.language.isoporpt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentInstituto de Ciências Biológicas - ICB (RG)pt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectPCRpt_BR
dc.subjectBovine DNApt_BR
dc.subjectSex determinationpt_BR
dc.subjectSensitivitypt_BR
dc.titleSensibilidade da PCR na amplificação do DNA bovino em diluição seriada e mistura de amostra macho e fêmeapt_BR
dc.title.alternativeSensitivity of PCR to amplify sex-specific bovine DNA in diluted samplespt_BR
dc.typeArtigopt_BR

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