Genotyping hepatitis C virus from hemodialysis patients in central Brazil by line probe assay and sequence analysis

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dc.creatorSanto, Marcia Paschoal do Espírito
dc.creatorCarneiro, Megmar Aparecida dos Santos
dc.creatorKozlowski, Aline Garcia
dc.creatorTeles, Sheila Araujo
dc.creatorLampe, Elisabeth
dc.creatorYoshida, Clara Fumiko Tachibana
dc.creatorMartins, Regina Maria Bringel
dc.date.accessioned2019-10-14T11:47:46Z
dc.date.available2019-10-14T11:47:46Z
dc.date.issued2007
dc.description.abstractThe present study examined the distribution of hepatitis C virus (HCV) genotypes and subtypes in a hemodialysis population in Goiás State, Central Brazil, and evaluated the efficiency of two genotyping methods: line probe assay (LiPA) based on the 5' noncoding region and nucleotide sequencing of the nonstructural 5B (NS5B) region of the genome. A total of 1095 sera were tested for HCV RNA by RTnested PCR of the 5' noncoding region. The LiPA assay was able to genotype all 131 HCV RNA-positive samples. Genotypes 1 (92.4%) and 3 (7.6%) were found. Subtype 1a (65.7%) was the most prevalent, followed by subtypes 1b (26.7%) and 3a (7.6%). Direct nucleotide sequencing of 340 bp from the NS5B region was performed in 106 samples. The phylogenetic tree showed that 98 sequences (92.4%) were classified as genotype 1, subtypes 1a (72.6%) and 1b (19.8%), and 8 sequences (7.6%) as subtype 3a. The two genotyping methods gave concordant results within HCV genotypes and subtypes in 100 and 96.2% of cases, respectively. Only four samples presented discrepant results, with LiPA not distinguishing subtypes 1a and 1b. Therefore, HCV genotype 1 (subtype 1a) is predominant in hemodialysis patients in Central Brazil. By using sequence analysis of the NS5B region as a reference standard method for HCV genotyping, we found that LiPA was efficient at the genotype level, although some discrepant results were observed at the subtype level (sensitivity of 96.1% for subtype 1a and 95.2% for subtype 1b). Thus, analysis of the NS5B region permitted better discrimination between HCV subtypes, as required in epidemiological investigations.pt_BR
dc.identifier.citationSANTO, M. P. Espírito et al. Genotyping of hepatitis C virus from hemodialysis patients in central Brazil by line probe assay and sequence analysis. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 40, n. 4, p. 545-550, Apr. 2007.pt_BR
dc.identifier.doi10.1590/S0100-879X2007000400013
dc.identifier.issne- 1414-431X
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/18221
dc.language.isoengpt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentFaculdade de Enfermagem - FEN (RG)pt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectHepatitis Cpt_BR
dc.subjectHepatitis C viruspt_BR
dc.subjectGenotypespt_BR
dc.subjectHemodialysispt_BR
dc.titleGenotyping hepatitis C virus from hemodialysis patients in central Brazil by line probe assay and sequence analysispt_BR
dc.typeArtigopt_BR

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