Cytoxicity and apoptotic mechanism of ruthenium(II) amino acid complexes in sarcoma-180 tumor cells

Página do item simplificado
dc.creatorLima, Aliny Pereira de
dc.creatorPereira, Flávia de Castro
dc.creatorAlmeida, Marcio Aurelio Pinheiro
dc.creatorAndrade, Francyelli Mariana dos Santos Mello
dc.creatorPires, Wanessa Carvalho
dc.creatorTeixeira, Thallita Monteiro
dc.creatorDelella, Flávia Karina
dc.creatorFelisbino, Sérgio Luis
dc.creatorMoreno, Virtudes
dc.creatorBatista, Alzir Azevedo
dc.creatorLacerda, Elisângela de Paula Silveira
dc.date.accessioned2018-08-28T11:44:13Z
dc.date.available2018-08-28T11:44:13Z
dc.date.issued2014-10
dc.description.abstractOver the past several decades, much attention has been focused on ruthenium complexes in antitumor therapy. Ruthenium is a transition metal that possesses several advantages for rational antitumor drug design and biological applications. In the present study, five ruthenium complexes containing amino acids were studied in vitro to determine their biological activity against sarcoma-180 tumor cells. The cytotoxicity of the complexes was evaluated by an MTT assay, and their mechanism of action was investigated. The results demonstrated that the five complexes inhibited the growth of the S180 tumor cell line, with IC 50 values ranging from 22.53 m M to 50.18 m M, and showed low cytotoxicity against normal L929 fibroblast cells. Flow cytometric analysis revealed that the [Ru(gly)(bipy)(dppb)]PF 6 complex (2) inhibited the growth of the tumor cells by inducing apoptosis, as evidenced by an increased number of Annexin V-positive cells and G0/G1 phase cell cycle arrest. Further investigation showed that complex 2 caused a loss of mitochondrial membrane potential; activated caspases 3, caspase-8, and caspase-9 and caused a change in the mRNA expression levels of caspase 3, caspase-9 as well as the bax genes. The levels of the pro-apoptotic Bcl-2 family protein Bak were increased. Thus, we demonstrated that ruthenium amino acid complexes are promising drugs against S180 tumor cells, and we recommend further investigations of their role as chemotherapeutic agents for sarcomas.pt_BR
dc.identifier.citationLIMA, Aliny Pereira; PEREIRA, Flávia Castro; ALMEIDA, Marcio Aurelio Pinheiro; MELLO, Francyelli Mariana Santos; PIRES, Wanessa Carvalho; PINTO, Thallita Monteiro; DELELLA, Flávia Karina; FELISBINO, Sérgio Luis; MORENO, Virtudes; BATISTA, Alzir Azevedo; SILVEIRA-LACERDA, Elisângela de Paula. Cytoxicity and apoptotic mechanism of ruthenium(II) amino acid complexes in sarcoma-180 tumor cells. Plos One, San Francisco, v. 9, n. 10, e105865, Oct. 2014.pt_BR
dc.identifier.doi10.1371/journal.pone.0105865
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/15760
dc.language.isoengpt_BR
dc.publisher.countryEstados unidospt_BR
dc.publisher.departmentInstituto de Ciências Biológicas - ICB (RG)pt_BR
dc.rightsAcesso Abertopt_BR
dc.titleCytoxicity and apoptotic mechanism of ruthenium(II) amino acid complexes in sarcoma-180 tumor cellspt_BR
dc.typeArtigopt_BR

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
Artigo - Aliny Pereira de Lima - 2014.pdf
Tamanho:
1.26 MB
Formato:
Adobe Portable Document Format
Baixar
Licença do Pacote
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Baixar

Coleções

ICB - Artigos publicados em periódicos