Doutorado em Ciência Animal (EVZ)
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Navegando Doutorado em Ciência Animal (EVZ) por Por Orientador "BRITO, Luiz Augusto Batista"
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Item Aspectos anatômicos do pênis, prepúcio e músculo retrator do pênis de bovinos das raças Gir e Nelore(Universidade Federal de Goiás, 2010-04-30) MENDONÇA, Alberto Corrêa; FIORAVANTI, Maria Clorinda Soares; http://lattes.cnpq.br/8772502020076257; SILVA, Luiz Antônio Franco da; http://lattes.cnpq.br/0446055905975647; BRITO, Luiz Augusto Batista; http://lattes.cnpq.br/3212353728321020Professionals of reproduction are increasingly looking for a deeper study of the penis and prepuce for the selection of reproductors to avoid factors that may favor the development of diseases causing impotence coeundi. In the surgical area this knowledgment facilitate nerve blocks and surgical approaches. So, it was aimed to conduct a review of penis and prepuce focusing embryological aspects, postnatal development, anatomical and histological composition, muscular origin and insertion, blood and lymphatic irrigation and drainage, innervation and mechanism of erection and ejaculation and to study morphometric aspects of the penis, prepuce and penis retractor muscle of Gir and Nelore cattle. In the first study, we selected 40 bulls aged between 30 to 38 months, being 20 Nelore and 20 Gir. These animals were evaluated by clinical examination and specific terms of the genital tract, test libido and mating ability. Later, the animals were carried to the slaughterhouse, where anatomical parts of the penis and preputium were collected in order to perform the morphometry and dissection of the retractor muscles of the penis, which, regardless of race presented in pairs in all animals. The second vertebra was the site of origin of the penis retractor muscle in all animals studied. The insertion of the left retractor muscle of the penis was more distally than the right one. However, there was no association with any disease process, since all animals showed themselves able of carrying out mating. In the second study, it was selected 23 Nelore and 20 Gir bulls from different centrals of collection of semen in order to study the morphometry and the correlation of different body and preputial measures. The average length of the prepuce of Nelore and Gir bulls was 52.4 5.5 cm and 57 3.2 cm, respectively. In the third study, for the penile morphometry, it was used the anatomical specimens of the first experiment. The penis length was, on average, 71.96 and 75.73 cm, respectively for the Nelore and Gir bulls. Some morphological and morphometric parameters quantified in this work, as the length of the prepuce, the shortest distance from the preputial orifice to the abdominal wall, lower distance from the umbilicus to the abdominal wall, circunference of the preputial orifice, length of the glans and length of free part of the penis, are found to be important parameters to evaluation criteria in the selection of reproductors for phenotypic characteristics improvement or even for prevention of acquired diseases that affect the external genitalia of bulls.Item Células-tronco da medula óssea e do tecido adiposo na regeneração do nervo ulnar em equinos(Universidade Federal de Goiás, 2012-08-17) MORAES, Júlia de Miranda; GODOY, Roberta Ferro de; http://lattes.cnpq.br/3912330933091811; MOURA, Veridiana Maria Brianezi Dignani de; http://lattes.cnpq.br/8773201078957745; BRITO, Luiz Augusto Batista; http://lattes.cnpq.br/3212353728321020The aim of this work was to evaluate the regeneration of equine ulnar nerves submitted to neurotomy, silicone tubing and cell therapy with bone marrow mononuclear cells fraction (MCF) or adipose derived mesenchymal stem cells (ADSC). Fifteen adult horses were divided into three groups with five animals each: control group (CG) with the use of saline solution, group with FCM deposition and group with ADSC deposition. The same surgical procedure was performed in all groups, using both nerves of each animal (right and left), establishing two moments of biopsy: on the 13th week on the right limb (CG1, MCF1 and ADSC1) and on the 26th week on the left limb (CG2, MCF2 and ADSC2). The MCF and ADSC were obtained respectively, from bone marrow and adipose tissue from each animal, both used as an authologous implant. After 13 and 26 weeks, biopsies were performed in all groups and immediately it was made some fragments slide imprints for viewing the nanocrystal fluorescent label. The fragments were fixed in 10% buffered formalin for histological analysis, with HE, luxol fast blue, Masson's trichrome, immunohistochemistry against the antibodies neurofilament (NF), S-100, FGF-2 and GDNF. Microscopically it was observed the presence of axonal growth, connective tissue, inflammatory infiltrate, Schwann cells, neural growth factors, myelin sheath and wallerian degeneration. For histologic analysis, it was established qualitative scores and for immunohistochemistry it was performed quantitative analysis with Image J program. It was observed wallerian degeneration reduction, better fascicular reorganization, new collagen increased, myelin sheath early formation, and NF antibody stronger staining in the experimental groups compared to CG. The ADSC group presented the best results than the other groups, showing ADSCs efficiency compared to MCF and CG for peripheral nerve regeneration. However, it was proved a longer time necessity to occur complete regeneration of peripheral nerve after injury.Item Desvio traumático de pênis em bovinos: aspectos epidemiológicos, morfofuncionais e tratamento cirúrgico empregando biomateriais(Universidade Federal de Goiás, 2009-04-30) RABELO, Rogério Elias; SILVA, Luiz Antônio Franco da; http://lattes.cnpq.br/0446055905975647; FIORAVANTI, Maria Clorinda Soares; http://lattes.cnpq.br/8772502020076257; BRITO, Luiz Augusto Batista; http://lattes.cnpq.br/3212353728321020