Programa de Pós-graduação em Ciências da Saúde
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Item Saturação de oxigênio pulpar em incisivos centrais superiores hígidos submetidos ao clareamento dentário: ensaio clínico randomizado(Universidade Federal de Goiás, 2018-03-16) Lima, Lorena Ferreira de; Estrela, Carlos; http://lattes.cnpq.br/3611967334176683; Alencar, Ana Helena Gonçalves de; http://lattes.cnpq.br/2039898015468283; Alencar, Ana Helena Gonçalves de; Estrela, Carlos; Baratto Filho, Flares; Silva, Julio Almeida daAim: To evaluate the oxygen saturation level (SaO 2 ) of the pulp of the central upper incisors, before, during and after the performance of the combined technique of toothbleaching by means of pulse oximetry. Materials and methods: This was a randomized, triple blind clinical trial, in which the initial sample consisted of 80 patients aged 18 to 27 years, 160 central incisors healthy upper randomly allocated into 4 groups: G1 - Office bleaching with two applications hydrogen peroxide (H 2 O 2 ) at 35% for 20 minutes each, followed by home bleaching with carbamide peroxide 10% for 2 hours a day for 16 days; G2 - Protocol used in G1, with the use of a desensitizer in the dentifrice; G3 - Office bleaching with application of H 2 O 2 at 35% and one placebo gel, both for 20 minutes, followed by application to home bleaching with carbamide peroxide 10% for 2 hours a day for 16 days; G4 - Protocol used in G3, with use of desensitizer in the dentifrice. The measurement at SaO 2 of the pulp was performed on the upper central incisors before (T0) and immediately after (T1) the office bleaching; in the 5 th (T2), 8 th (T3), 12 th (T4) and 16 th day of home bleaching (T5); and after 7 (T6) and 30 (T7) days of completion of procedures. The mean level of SaO 2 pulp was described by mean and standard deviation. times and the average values of saturation in groups by Estimations Generalized Equations model were compared (GEE) and used the Student t test for paired samples for analysis of overall average initial time (T0) and after thirty days after the end of tooth bleaching (T7) (p <0.05). Outcomes: The final sample consisted of 60 patients (n = 120 teeth), G1 (n = 28), G2 (n = 40), G3 (n = 26) and G4 (n = 26). The results showed a mean pulp SaO 2 level at G0 of 84.29%, G2 of 84.38%, G3 of 84.79% and G4 of 85.83%. There was a reduction of T0 for T1, 81.96%, 82.06%, 82.19% and 81.15% for G1, G2, G3 and G4, respectively, with difference in G4 (p = 0.006). During home bleaching, there was returned of the pupal oxygen saturation level in all groups, with mean values in T7 of 86.55%, 86.60%, 85.71%, 87.15% for G1, G2, G3 and G4, respectively, with difference in G2 (p = 0.004). Conclusion: The average level of initial pulp oxygen saturation for central upper incisors was 84.76%, with changes in saturation during the combined technique of tooth bleaching and final mean level, after 30 days, of 86.52%.Item Avaliação da saturação de oxigênio em polpas humanas de molares hígidos(Universidade Federal de Goiás, 2016-03-08) Oliveira, Keila Surama Alves de; Estrela, Carlos; http://lattes.cnpq.br/3611967334176683; Alencar, Ana Helena Gonçalves de; http://lattes.cnpq.br/2039898015468283; Alencar, Ana Helena Gonçalves de; Estrela, Carlos; Decúrcio, Daniel de AlmeidaObjective: to determine the oxygen saturation level (SaO2) in human pulps of molars by pulse oximetry. Methods: the oxygen saturation level was evaluated in 112 healthy molars using the pulse oximeter and the patient's response time to stimulus with the cold refrigerant gas Endo Ice and recorded with digital timer. Statistical analysis was performed using SPSS v program. 18.0. Quantitative variables were described by mean and standard deviation when the distribution was symmetric and median and interquartile range when asymmetric. Variables with symmetric distribution were compared between teeth for independent samples and intra-individual for paired samples by Student t test, and the asymmetric distribution with the Mann-Whitney test. To correlate the variables each other was used the Pearson correlation coefficient, and to compare more than two groups together the analysis of variance (ANOVA) followed by post-hoc Tukey, being statistically significant p <0,05. Results: the average level of SaO2 for the 112 pulps of healthy molars was 85,09%, and there was no correlation with the SaO2 average of the patient´s indicator finger (92,89%). There was a significant difference (P = 0,037) between the average level of SaO2 of the first (85,76%) and second superior molars (81,87%), and it was not significant (P = 0,177) between the first (85,58%) and second (88,15%) inferior molars. The superior molars had lower average level of SaO2 (83,59%) when compared to the inferior molars (86,89%), with a statistically significant difference (P = 0,018). The average of the patient's response time to the cold stimulus was 1,12 seconds, with no statistically significant difference between superior (1,25 seconds) and inferior molars (0,99 seconds). Conclusion: the average level of SaO2 in healthy molars pulps was 85,09%, and the average of the superior molars was 83,59% and inferior molars was 86,89%. The average of the patient's response time to the cold stimulus was 1,12 seconds, with no statistically significant difference between superior (1,25 seconds) and inferior molars (0,99 seconds) and there was no correlation between the patient's response time to the cold stimulus and the oxygen saturation level for healthy molars.Item Análise comparativa da resistência de união e à compressão de cimentos biocerâmicos utilizados em procedimentos de endodontia regenerativa(Universidade Federal de Goiás, 2021-01-12) Rodrigues, Maykely Naara Morais; Bruno, Kely Firmino; http://lattes.cnpq.br/0680297602065791; Alencar, Ana Helena Gonçalves de; http://lattes.cnpq.br/2039898015468283; Alencar, Ana Helena Gonçalves de; Decurcio, Daniel de Almeida; Estrela, CarlosObjective: To compare the bond strength to root dentin by means of the mechanical push-out test and the compressive strength of BIODENTINE®, MTA REPAIR HP® and BIO-C REPAIR® bioceramic cements. Materials and methods: For the test of bond strength to root dentin, 18 uniradicular lower premolars were used, sectioned 5.0 mm below the cemento-enamel junction (JCE). Cervical preparations were performed with a Largo # 1, # 2, # 3, # 4, # 5 and # 6 drill and the intracanal medication used was the antibiotic double paste, which remained in the root canal for 21 days. After removing the intracanal medication, the roots were cross-sectioned to obtain 1 mm thick dentin discs, consisting of 3 samples per tooth. Next, the 54 dentin discs were randomly divided into 3 experimental groups (n = 18) for insertion of the following bioceramic cements: Group 1 - BIODENTINE®; Group 2 - MTA REPAIR HP®; and, Group 3 - BIO-C REPAIR®. Cements were handled according to the manufacturers' recommendations and inserted into the root canal of each specimen placed on a glass plate, and subsequently kept in an oven at 37ºC and 100% relative humidity for 7 days. After the storage period, the mechanical push-out test was performed and the specimens were analyzed in the stereomicroscope with a 3x magnification to determine the failure pattern. For the compressive strength test, specimens (n = 10) of each of the bioceramic cements evaluated were made, with dimensions of 6 mm in height x 3 mm in diameter, these were stored in an oven at 37 ° C and 100% relative humidity for 7 days. After the storage period, the specimens were taken to the test device of the Universal Testing Machine to perform the test. The data were analyzed using the Shapiro-Wilk normality test. In the mechanical push-out test, the Kruskal-Wallis non-parametric test was used and multiple comparisons were performed using the Friedman test, with the failure pattern presented as a percentage. In the compressive strength test, the ANOVA test was used and multiple comparisons were performed using the Tukey test. The level of significance was set at 0.05%. Results: For the bond strength to root dentin, the bioceramic cement BIODENTINE® performed better than MTA REPAIR HP® and BIO-C REPAIR®, however, the difference was only statistically significant between the BIODENTINE® and BIO-C groups. REPAIR® (p = 0.0001). In the BIODENTINE® group, the most frequent failure pattern was mixed (61%), to the detriment of cohesive (33%) and adhesive failures (6%). On the other hand, in the MTA REPAIR HP® group, adhesive failures (94%) and only (6%) of mixed failures were observed more frequently, with no cohesive failures. Likewise, the BIO-C REPAIR® group did not present cohesive failures, but showed a higher prevalence of adhesive failures (72%) and a greater number of mixed failures (28%) when compared to MTA REPAIR HP®. With respect to compressive strength, the bioceramic cement BIODENTINE® showed greater resistance to compression than MTA REPAIR HP® and BIO-C REPAIR®, this difference being statistically significant (p <0.05). Conclusion: Bioceramic cement BIODENTINE® showed higher values of bond strength to root dentin and compressive strength, however for the push-out test this difference was statistically significant only for BIO-C REPAIR® cement.Item Determinação da espessura cemento-dentina remanescente em zona de perigo de molares inferiores após preparo do canal radicular usando novo software de tomografia computadorizada de feixe cônico(Universidade Federal de Goiás, 2020-06-29) Sousa, Vinícius Caixeta de; Estrela, Carlos; http://lattes.cnpq.br/3611967334176683; Alencar, Ana Helena Gonçalves de; http://lattes.cnpq.br/2039898015468283; Estrela, Carlos; Guedes, Orlando Aguirre; Decurcio, Daniel de Almeida; Porto, Olavo Lyra; Barletta, Fernando BrancoThis study measured the thickness of cementum/dentin in the danger zone of mandibular molars after root canals preparation (RCP) using a novel cone-beam computed tomography (CBCT) software. Material and methods: Eighty-four teeth were distributed into four groups: G1. ProTaper Next; G2. BioRace; G3. Reciproc Blue; G4. WaveOne Gold. CBCT images were acquired before and after RCP. The e-Vol DX® CBCT software was used to measure initial and final remaining cementum-dentin thickness after RCP of the mesial root of mandibular molars at 1 and 3 mm from the furcation. The Kolmogorov Smirnov test, analysis of variance and Student t test were used to statistical analysis. The level of significance was set at 5%. Results: Differences between mean initial and final thicknesses of the mesiobuccal (MB) and mesiolingual (ML) canals were not statistically significant. Lower mean initial thickness at 3 mm (0.900mm ± 0.191) than at 1 mm (1.035mm ± 0.184) indicates that this is the danger zone. Although cementum/dentin is thinner at 3 mm from the furcation (0.715 ± 0.186) after RCP, the greatest amount of dentin removal was found at 1 mm (0.734 ± 0.191). Conclusions: Remaining cementum dentin was thicker than 0.715 mm in root canals prepared using #35 (WaveOne Gold®) and #40 (ProTaper Next®, BioRace® and Reciproc Blue®) instruments, which confirms the safety of canal preparation in the danger zone.Item Efeito antibacteriano dos sistemas Self-Ajusting File, XP-endo finisher e irrigação ultrassônica passiva sobre biofilme de Enterococcus faecalis(Universidade Federal de Goiás, 2016-04-19) Sousa, Vinicius Caixeta de; Estrela, Carlos; http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4792692Y0; Alencar, Ana Helena Gonçalves de; http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4792653A9; Alencar, Ana Helena Gonçalves de; Estrela, Carlos; Decurcio, Daniel de Almeida; Silva, Julio Almeida; Sousa, Hugo Alexandre deAbstract: Objective: To evaluate the effectiveness of complementary protocols of sanitization in the decontamination of infected root canals. Methods: Fifty uniradicular maxilary anterior human teeth were selected. The crowns were removed and the lengths standardized at 16 mm. The specimens were inoculated with Enterococcus faecalis and incubated at 37 °C for sixty days. Thirty teeth were performed with BioRace instruments until diameter corresponding to #60.02, and then complemented with Self-Adjusting File (SAF); XP-endo Finisher (XPF) and passive ultrasonic irrigation (PUI). Ten samples were used as positive control and 10 were not contaminated. Initial and final samples were collected and incubated at 37 °C for a period of 48 hours. The bacterial growth was analyzed in culture, determining the presence or absence of bacteria. The optical density of the culture medium was interpreted by UV spectrophotometry. The specimens were sectioned and prepared for evaluation in SEM. The images of the root surfaces were analyzed and classified by scores according to the presence of debris. The Kruskal-Wallis test was used for statistical analysis. The level of significance was 5%. Results: The mean optical density (μm) of the sanification protocols showed bacterial reduction in all groups. The experimental groups did not present statistically significant differences (p = 0.196). The analysis of SEM images revealed no significant difference (p = 0.414) between the scores of the groups. Conclusion: Complementary sanitization protocols reduced bacterial contamination.