Influência da exposição ao carbonato de cálcio no metabolismo de compostos orgânicos e inorgânicos em biomphalaria glabrata, hospedeiro de schistosoma mansoni

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2014-03-26

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Universidade Federal de Goiás

Resumo

Biomphalaria snails act as intermediate hosts that harbor the Schistosoma mansoni parasite, the causative agent of schistosomiasis. As population increases, snails have their geographical distribution conditioned to the presence of calcium in the environment, which is essential for their energetic metabolism. The objective of this study was the assessment on the influence of exposure to different concentrations of CaCO3 (20, 40, 60, 80 and 100 mg/L) at different intervals (1, 14, 21 and 30 days) forthe concentrations of glucose, calcium, total proteins, urea, uric acid and creatinine in hemolymph B. glabrata. All substances were dosed using specific kits with a spectrophotometer reading at 545 nm. High performance liquid chromatography (HPLC) was the technique applied to quantify the presence of organic acids: pyruvate, oxaloacetate, citrate, succinate, fumarate, propionate, acetoacetate and βhydroxybutyrate in the hemolymph of mollusks exposed to CaCO3. Results revealed that the calcium concentration in the hemolymph did not present significant difference (p>0.05) with regard to control as well as to the concentrations assessed. The glucose concentration decreased (p<0.05) in exposures to 20mg and 40mg and increased in exposures to 80mg and 100mg of CaCO3 with regard to control. The organic acids pyruvate, oxaloacetate, citrate, succinate fumarate and lactate had their concentrations increased; propionate in turn had its concentration decreased set against control in exposure to CaCO3. In the exposures to the concentrations assessed, acetoacetate decreased and β-hidroxibutirato increased. Total proteins and urea presented decrease. The uric acid concentration increased in the exposure to 20mg of CaCO3; the creatinine concentration increased in the exposures to 40mg and 100mg of CaCO3. It was possible to conclude that snails exposed to different concentrations of CaCO3 had their major metabolic alterations occurring from the 14th day of exposure.

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Silva, Luciana Damacena. Influência da exposição ao carbonato de cálcio no metabolismo de compostos orgânicos e inorgânicos em biomphalaria glabrata, hospedeiro de schistosoma mansoni. 2014. 90 f. Tese (Doutorado em Medicina Tropical e Saúde Pública) - Programa de Pós-graduação em Medicina Tropical e Saúde Publica (IPTSP) - Universidade Federal de Goiás, Goiânia, 2014.