Detecção de anticorpos para arbovirus em primatas não humanos no município de Goiânia, Goiás
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2015-06-29
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Universidade Federal de Goiás
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Arboviruses are viruses that maintained in nature by passing through an arthropod vector to a susceptible vertebrate and non-human primate (NHP), an important host, mainly in wild environment. This study aimed to identify antibodies for arboviruses in NHPs inhabitants of three urban parks of Goiania city, as well as NHPs acclimatized the Wild Animal Screening Center - CETAS-IBAMA, Goiania city. The procedure took place from the capture and collection of blood samples from 50 animals of species Cebus libidinosus (n = 42), Alouatta caraya (n = 5) and Callithrix penicillata (n = 3). All serum samples were first subjected to hemagglutination inhibition assay (HI) against specific antigens of 23 types of arboviruses circulating in the Brazilian Amazon region having as revelation goose red blood cell system. It was observed a positivity in serum samples of six NHPs, three of them being of the species Cebus libidinosus (animals 02, 10 and 11), two of Alouatta caraya species (animal 12 and 20) and the others for Callithrix penicillata species (animal 23) These six animals, four of them showed seropositive for more than one type of arboviruses: Animal 02 (Cacipacore virus and Dengue-1); Animal 11 (Mayaro virus, Oropouche, Cacipacore, Bussuquara and Dengue-1, 3 e 4); Animal 12 (Oropouche virus, Bussuquara and Cacipacore); and animal 23 (Dengue- 1, 3 and 4). The other two NHPs, species Cebus libidinosus (animal 10) and Alouatta caraya (animal 20) were seropositive for only one type of arbovirus: Bussuquara and Oropouche, respectively. Considering antibody titration by HI, it was observed a titer of equal or greater than 1:40 for Cacipacore virus and Dengue-1 (animal 02), Bussuquara (animal 10) and Oropouche (animals 11, 12 and 20), in total of five serum samples. Of these, four were tested for serum neutralization test (SNT) in vivo using Swiss mice of 1-3 days old: sample of animal 02, was positive for the virus Cacipacore and samples of animals 11, 12 and 20 were positive for Oropouche virus. It was noted that two of these samples was confirmed as positive for Oropouche virus from animals 12 and 20, both of Allouatta caraya species, which were acclimatized at CETAS-IBAMA. Generally, the study showed seropositivity for seven types of arboviruses in the three species studied NHPs which are registered in the Cerrado: Cebus libidinosus, Allouatta caraya and Callithrix penicillata.
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GIBRAIL, Marize M. Detecção de anticorpos para arbovirus em primatas não humanos no município de Goiânia, Goiás. 2015. 64 f. Dissertação (Mestrado em Medicina Tropical e Saúde Publica) - Universidade Federal de Goiás, Goiânia, 2015.