Metabolismo do acetato entre membros do gênero paracoccidioides spp
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2017-04-13
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Universidade Federal de Goiás
Resumo
Members of the genus Paracoccidioides are known pathogens of humans that can be isolated from
different infection sites. To investigate the expression rates of proteins expressed by different
isolates, of the genus Paracoccidioides, including one isolate of P. lutzii (Pb01), and three isolates
of P. brasiliensis (Pb03, Pb339 and PbEPM83) using sodium acetate, as carbon source, proteins
quantities were determined using label-free and data independent LC-MSE. Statistical analysis
provided the comparison of proteins profiles in the isolates. A total of 1160, 1211, 1280 and 1462
proteins were reproducibly identified, and relatively quantified in P. lutzii e P. brasiliensis isolates
Pb03, Pb339 e PbEPM83, respectively. Notably, a total of 526, 435, 744 and 747 proteins were
differentially expressed among P. lutzii and the P. brasiliensis isolates Pb03, Pb339 and PbEPM83,
respectively with a fold change equal or higher than 1.5. The analysis revealed the reorganization of
metabolism through the induction of proteins related to gluconeogenesis, stress response and amino
acid degradation in the four isolates evaluated. The differences between the isolates were observed
as follows: greater increases in the expression levels of proteins belonging to the cycle of glyoxalate,
TCA and respiratory chain, ethanol production and β-oxidation were observed in the
isolatesPPEPM83 and Pb01; Cyclic and cyclocyclic proteins of the methylcitrate were induced in
Pb339. Proteomic profiles indicate that the four isolates reorganize the metabolism for the use of
acetate as a carbon source.
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MATA, F. R. Metabolismo do acetato entre membros do gênero paracoccidioides spp. 2017. 200 f. Tese (Doutorado em Genética e Biologia Molecular) - Universidade Federal de Goiás, Goiânia, 2017.