Utilização do extrato etanólico de jabuticaba (Myrciaria cauliflora) no controle de Salmonella Heidelberg na produção de frangos de corte
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2018-05-23
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Universidade Federal de Goiás
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The objective of the present study was to evaluate the use of the ethanolic extract of of bark
and seeds of Myrciaria cauliflora in the incubation of fertile eggs and in the feeding of broilers
inoculated with Salmonella Heidelberg, observing possible beneficial and or protective effects
of this phytogenic on health systemic effects of birds. Three experiments were carried out at
the Experimental Center for Bird Diseases and Laboratory of Bacteriology of the Department
of Veterinary Medicine of the Veterinary and Zootechnical School of the Federal University of
Goiás. In the first experiment was evaluated the antimicrobial in vitro activity was evaluated
and the minimum inhibitory concentration of ethanolic extract of M. cauliflora on Salmonella
enterica different strains. In the second experiment, 320 fertile eggs distributed in randomized
design were used in four treatments with 10 replicates each: CN - negative control group; EJ -
group that received only the extract in the shell; SH - group that received only the bacterial
inoculum in the shell; and SH + EJ - group that received the bacterial inoculum and the extract
in the shell. The eggs incubated non-hatched were submitted to embryodiagnosis and
processed for Salmonella in the embryos. The chicks born were housed until 10 days of age,
during which time the collection and processing of samples for Salmonella research, serum
biochemistry and hemogram were performed. In the third experiment, 336 one - day - old male
chicks were distributed in randomized design in four treatments with seven replicates each: CN
- negative control group; EJ - group that received only the vegetal extract in the diet; SH -
group that received the inoculum bacterial in crop; and SH + EJ - group that received the
bacterial inoculum in crop and the extract in the diet. Performance indexes, fecal excretion and
presence of Salmonella in organs, organ biometry, intestinal histomorphometry, goblet cell
counts and immune cells in organs and blood were evaluated. In the first experiment, the
extract presented low inhibitory action on the strains of Salmonella enterica,, Salmonella
Typhi, Salmonella Heidelberg and Salmonella Typhimurium. In the second experiment it was
verified that the extract promoted less hatchability and higher embryonic mortality. The
Salmonella isolation was higher in the embryo and meconium samples of the group that
received the extract. At 10 days of age the extract reduced the excretion and colonization of the
organs by Salmonella and the hematocrit and serum cholesterol level. In the third experiment it
was verified that the extract increased the excretion of Salmonella in the inoculated birds and
reduced the number of lymphocytes in the blood. It is concluded that the use of the ethanolic
extract of bark and seeds of M. cauliflora presented weak antimicrobial activity in vitro for the
strains of Salmonella enterica tested. In embryonated eggs its use is not feasible, however
when added in the diet, it can stimulate the production of lymphocytes in lymphoid organs.
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ARAÚJO-LEONÍDIO, A. R. Utilização do extrato etanólico de jabuticaba (Myrciaria cauliflora) no controle de Salmonella Heidelberg na produção de frangos de corte. 2018. 115 f. Tese (Doutorado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2018.