Aspectos moleculares da interação patogênica entre Sclerotinia sclerotiorum e Phaseolus vulgaris L.
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2016-02-26
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Universidade Federal de Goiás
Resumo
White mold caused by Sclerotinia sclerotiorum (Lib.) De Bary is one of the most devastating
diseases of common bean (Phaseolus vulgaris L.) and others crops. This study aimed to achieving a better understanding of the pathosystem S. sclerotiorum-P. vulgaris identifying genes potentially involved in this interaction. Two different approaches were used to evaluate this pathosystem: 1) susceptible cultivar Pérola (BRS) of P. vulgaris; and 2) treatment of this cultivar with the resistance inducer methyl jasmonate (MeJA). For both situations, a suppression subtractive hybridization (SSH) cDNA library approach was used to identify differentially expressed genes in the pathogen and the host plant. In the interaction between the susceptible cultivar and S. sclerotiorum 979 unigenes (430 contigs and 549 singletons) were inentified with 29% of these unigenes were categorized as fungal genes, related to cell wall degradation, proteins degradation, oxidative stress among other. And 58,3% were categorized as plant genes related to energetic metabolism, protein degradation, response to biotic and abiotic stress. The selection of differential clones of their libraries SSH has been validated by reverse transcription and quantitative real-time PCR (RT-qPCR). Were validated genes related to pathogenicity and virulence factors of S. sclerotiorum, such as genes encoding enzymes that degrade plant cell walls (polygalacturonases, cellulases, hemicellulases and proteases). And besides these, defense-related genes of the host plant as: pathogenesis-relateds genes (PR1, PR2, and PR3), phenylpropanoid pathway (Isof, FPS1, and 4CL), and involved in defense and stress-related categories (Lox, Hiprp, GST, POD, and DOX). Exogenous MeJA application induced partial resistance agains S. sclerotiorum in plants as well as a consistent increase in pathogenesis- related protein activities. Furthermore, the validation of SSH made by RT-qPCR showed differential expression of important unigenes over time, such as: pathogenesis related protein PR3 (chitinase class I), PvCallose (callose synthase), PvNBS-LRR (NBS-LRR resistance-like protein), PvF-box (F-box family protein-like), and a polygalacturonase inhibitor protein (PGIP). The data obtained in this study have highlighted some of the mechanisms involved in the pathogenicity of the fungus, as well as defense responses produced by the plant in two conditions evaluated, thus contributing to a better understanding of pathosystem.
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OLIVEIRA, M. B. Aspectos moleculares da interação patogênica entre Sclerotinia sclerotiorum e Phaseolus vulgaris L. 2016. 115 f. Tese (Doutorado em Biologia) - Universidade Federal de Goiás, Goiânia, 2016.